Genetic purity analysis of cotton (Gossypium spp.,) hybrids using SSR markers

Seed Science and Technology (Impact Factor: 0.7). 07/2010; 38:358-366. DOI: 10.15258/sst.2010.38.2.09

ABSTRACT Use of morphological differences, between true hybrids and off types in grow out test (GOT) for genetic purity analysis, are not always apparent and cannot be recognised easily. Further, morphological traits are costly, tedious to score and environment sensitive. Alternatively, it is suggested that recent breakthrough in molecular markers can be employed in genetic purity analysis. The genetic purity of three cotton hybrids (TCHB 4510, TCHB 2310 and TCHB 213) that are widely cultivated in Tamil Nadu, India were assessed by GOT and molecular markers. A total of 400 individuals from each one of the three hybrids were raised in the field and morphological traits were recorded. Results of this GOT have shown that TCHB 2310 had lowest genetic purity (62.5%) followed by TCHB 4510 (78.2%) and TCHB 213 (95.2%). Simple sequence repeats (SSR) marker analysis of parents that were involved in the production of all the three hybrids have shown that 45 out of 150 SSRs were polymorphic among the parents. From this set of polymorphic SSRs, BNL686, BNL1679, BNL3971, BNL3955, CIR407 and CIR413 were selected to test the genetic purity of hybrid seeds since they have produced clear, scorable and unambiguous polymorphic bands among the parents. All the three hybrids were clearly distinguished from their selfed females and off types using these six SSRs. Hence, it is proposed that these SSR markers can be used in efficient analysis of hybrid seed purity since this technique is simple to use, more accurate and not affected by environment when compared with GOT.

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    ABSTRACT: Narrow genetic base and complex allotetraploid genome of cotton (Gossypium hirsutum L.) is stimulating efforts to avail required polymorphism for marker based breeding. The availability of draft genome sequence of G. raimondii and G. arboreum and next generation sequencing (NGS) technologies facilitated the development of high-throughput marker technologies in cotton. The concepts of genetic diversity, QTL mapping, and marker assisted selection (MAS) are evolving into more efficient concepts of linkage disequilibrium, association mapping, and genomic selection, respectively. The objective of the current review is to analyze the pace of evolution in the molecular marker technologies in cotton during the last ten years into the following four areas: (i) comparative analysis of low-and high-throughput marker technologies available in cotton, (ii) genetic diversity in the available wild and improved gene pools of cotton, (iii) identification of the genomic regions within cotton genome underlying economic traits, and (iv) marker based selection methodologies. Moreover, the applications of marker technologies to enhance the breeding efficiency in cotton are also summarized. Aforementioned genomic technologies and the integration of several other omics resources are expected to enhance the cotton productivity and meet the global fiber quantity and quality demands.
    The Scientific World Journal 10/2014; 2014. · 1.73 Impact Factor


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