Genome Sequence of Bacillus licheniformis WX-02

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, People's Republic of China.
Journal of bacteriology (Impact Factor: 2.81). 07/2012; 194(13):3561-2. DOI: 10.1128/JB.00572-12
Source: PubMed


Bacillus licheniformis is an important bacterium that has been used extensively for large-scale industrial production of exoenzymes and peptide antibiotics. B. licheniformis WX-02 produces poly-gamma-glutamate increasingly when fermented under stress conditions. Here its genome sequence (4,270,104 bp, with G+C content of 46.06%), which comprises a circular chromosome, is announced.

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Available from: Gaofu Qi, Apr 18, 2014
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    • "Experiments were performed with the strains and plasmids listed in Table 1. The oligonucleotide primers listed in Table 2 were designed on the basis of B. licheniformis WX-02 genome sequence [GenBank: AHIF00000000] [16]. Luria-Bertani (LB) medium was prepared for culture of E. coli DH5α and also B. licheniformis[23]. "
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    • "PGA is mainly found as a capsular polymer; therefore, its accumulation as an exo-polymer, as observed for B. subtilis and B. licheniformis, the PGA synthetic system of which is virtually the same as that of B. subtilis (Wang et al., 2011; Yangtse et al., 2012), is currently considered a peculiar phenomenon. Comparative genetic analysis of the pgs and cap operons (Fig. 5) reveals a difference in their downstream genes for PGA cleavage. "
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