MiR-297 modulate multidrug resistance in human colorectal carcinoma by down-regulating MRP-2

State Key Laboratory of Bioreactor Engineering and Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, PR China.
Biochemical Journal (Impact Factor: 4.4). 06/2012; 446(2):291-300. DOI: 10.1042/BJ20120386
Source: PubMed


Colorectal carcinoma is a frequent cause of cancer-related death in men and women. miRNAs (microRNAs) are endogenous small non-coding RNAs that regulate gene expression negatively at the post-transcriptional level. In the present study we investigated the possible role of microRNAs in the development of MDR (multidrug resistance) in colorectal carcinoma cells. We analysed miRNA expression levels between MDR colorectal carcinoma cell line HCT116/L-OHP cells and their parent cell line HCT116 using a miRNA microarray. miR-297 showed lower expression in HCT116/L-OHP cells compared with its parental cells. MRP-2 (MDR-associated protein 2) is an important MDR protein in platinum-drug-resistance cells and is a predicted target of miR-297. Additionally miR-297 was down-regulated in a panel of human colorectal carcinoma tissues and negatively correlated with expression levels of MRP-2. Furthermore, we found that ectopic expression of miR-297 in MDR colorectal carcinoma cells reduced MRP-2 protein level and sensitized these cells to anti-cancer drugs in vitro and in vivo. Taken together, our findings suggest that miR-297 could play a role in the development of MDR in colorectal carcinoma cells, at least in part by modulation of MRP-2.

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    • "ABCC2 can recognize GSH-conjugated form of Pt drugs and effectively pump them out of the cells to confer resistance. To date, only miR-297 has been reported to be down-regulated in a oxaliplatin-resistant colon cancer cell model (HCT116/L-OHP) to cause ABCC2 overexpression and Pt drug resistance [31]. A complementary binding site for miR-297 was identified on ABCC2 3′UTR to mediate the specific gene repression. "
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