Article

HIF-1α protein is upregulated in HIF-2α depleted cells via enhanced translation.

Institute of Biochemistry I, Faculty of Medicine, Goethe-University Frankfurt, 60590 Frankfurt, Germany.
FEBS letters (impact factor: 3.54). 06/2012; 586(11):1652-7. DOI:10.1016/j.febslet.2012.04.039
Source: PubMed

ABSTRACT The hypoxia-inducible factors HIF-1 and HIF-2 are primarily regulated via stabilization of their respective ?-subunits under hypoxic conditions. Previously, compensatory upregulation of one HIF-α-subunit upon depletion of the other α-subunit was described, yet the underlying mechanism remained elusive. Here we provide evidence that enhanced HIF-1α protein expression in HIF-2α knockdown (k/d) cells neither results from elevated HIF-1α mRNA expression, nor from increased HIF-1α protein stability. Instead, we identify enhanced HIF-1α translation as molecular mechanism. Moreover, we found elevated levels of the RNA-binding protein HuR and provide evidence that HuR is critical for the compensatory HIF-1α regulation in HIF-2α k/d cells.

0 0
 · 
1 Bookmark
 · 
61 Views
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

-subunits
 
compensatory HIF-1α regulation
 
compensatory upregulation
 
depletion
 
elusive
 
enhanced HIF-1α protein expression
 
HIF-1α mRNA expression
 
HIF-1α translation
 
HIF-2
 
HIF-2α k/d cells
 
HIF-2α knockdown
 
HIF-α-subunit
 
hypoxia-inducible factors HIF-1
 
RNA-binding protein HuR
 
α-subunit
 

Tobias Schmid