Immunohistochemical localization of CD1a and S100 in gingival tissues of healthy and chronic periodontitis subjects.
ABSTRACT Oral Diseases (2012) 18, 778-785 Objective: The aim of this study was to evaluate the presence and distribution of CD1a and S100 protein markers in states of gingival health and chronic periodontitis in human subjects. Materials and Methods: Gingival tissue samples were derived from 10 healthy and 10 chronic periodontitis-affected human subjects. The presence and distribution of CD1a and S100 protein was assessed using immunohistochemistry, and the cell types involved in their expression was determined. Results: The presence and distribution of CD1a was confined only to the gingival epithelium, whereas S100 was seen in the epithelium and connective tissue. However, increased expression of both CD1a and S100 protein was seen in periodontitis-affected gingival tissues compared with healthy gingiva. Immunohistochemistry demonstrated that CD1a- and S100-positive cells in the epithelium are Langerhans cells (LCs) and S100 positive cells in the connective tissue are dendritic cells (DCs). Conclusion: Our findings suggest the transition of CD1a-positive LCs to S100-positive DCs from epithelium to connective tissue in response to an antigenic challenge. Demonstration of increased number of S100-positive DCs in the gingival connective tissue in chronic periodontitis possibly suggests their involvement in bone resorption in addition to their antigen presentation property.
- SourceAvailable from: Ricardo Alves Mesquita[Show abstract] [Hide abstract]
ABSTRACT: Aim: To evaluate the relationship between chemokines and dendritic cells (DCs) in human chronic periodontitis (CP). Material and Methods: Gingival samples were obtained from 23 individuals with human chronic periodontitis and six samples of normal mucosa (NM) overlapping the third molar were used to control the chemokine levels. Periodontal examination was conducted. Immunohistochemistry was performed for Factor XIIIa+ and CD1a+ immature DCs, and CD83+ mature DCs. The levels of the chemokines CCL2, CCL3, CCL5, CCL19, CCL20, and CXCL8 were measured in gingival tissues using enzyme-linked immune sorbent assay (ELISA). Inflammatory infiltrate, DCs, chemokines, classification of human chronic periodontitis, and clinical parameters were correlated and compared. Results: The expression of CCL2 and CCL20 was positively correlated with increased densitites of CD1a+ DCs. CCL3 and CXCL8 were positively related to the clinical attachment level. The CCL3, CCL5, CCL19, and CXCL8 levels increased in the gingival samples of chronic periodontitis patients when compared to NM, while CCL20 levels increased in advanced CP as compared to mild-moderate CP. Conclusion: An increased number of CD1a+ immature DCs is related to CCL2 and CCL20. CCL3 and CXCL8 chemokines are related to a greater severity of human chronic periodontitis.Journal of Periodontology 03/2014; · 2.57 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: There are a limited number of studies correlating the different stages of dendritic cells (DC) maturation with cytokines in individuals presented chronic periodontitis (CP). The aim of the study was to evaluate the correlation among the expression of IL-2, IL-10, IL-4, IL-6, IFN-[Formula: see text], TNF-α, and IL-17A with the presence of DC and mild-moderate or advanced CP. Gingival samples were obtained from 24 individuals with CP and six samples of normal mucosa (NM) overlapping third molar for controls of the levels of cytokines. Periodontal examination was performed. Immunohistochemical staining was carried out, revealing CD1a+ immature, Fator XIIIa+ immature, and CD83+ mature DCs. The inflammatory infiltrate was counted, and the cytokines were measured by flow cytometry. Densities of DCs and inflammatory infiltrate, cytokines, subtypes of CP, and clinical periodontal parameters were correlated and compared. IL-6 expression was correlated positively with the increased numbers of CD1a+ immature DCs. Levels of IL-2, TNF-α, IFN-[Formula: see text], IL-10, and IL-17A were increased when compared with NM. The percentage of sites with clinical attachment level (CAL)>3 were positively correlated with densities of inflammatory infiltrate and negatively correlated with densities of immature DCs. IL-6 can contribute to the increase of the immature DCs in the CP. Higher levels of IL-2, TNF-α, IFN-[Formula: see text], IL-10, and IL-17A cytokines were observed in CP. Higher densities of inflammatory infiltrate as well as lower densities of immature DCs can result in a more severe degree of human CP.PLoS ONE 01/2014; 9(3):e91636. · 3.53 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: The chronic periodontitis and psoriasis share common risk factors and co-morbidities. This study was designed to explore how frequently chronic periodontitis (CP) is associated with psoriasis compared to systemic healthy subjects MATERIAL AND METHODS: The periodontal clinical parameters probing depth (PD), periodontal attachment level (PAL) and presence or absence of radiographic bone loss (Page & Ese, 2007) were recorded in 33 psoriasis subjects and 35 healthy subjects. The severity of psoriasis was assessed and recorded by Psoriasis Area and Severity Index (PASI) as described earlier by Cohen et al (Cohen et al, 2005). The other oral health characteristics were also recorded.Oral Diseases 07/2014; · 2.40 Impact Factor