Water-Soluble Conjugated Polymers for Imaging, Diagnosis, and Therapy

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.
Chemical Reviews (Impact Factor: 46.57). 06/2012; 112(8):4687-735. DOI: 10.1021/cr200263w
Source: PubMed


Water-soluble conjugated polymers (WSCP) for imaging, diagnosis, and therapy, and the progress in the recent 5 years, from 2007 to 2011, is discussed. Liu and co-workers synthesized α-mannose and β-glucose containing PTs and PFPs. To overcome the relative instability of liquid-phase PDA sensors, Park and co-workers developed a solid-phase chip based on streptavidin-functionalized PDA liposome for the diagnosis of pathogen infection. Shi and co-workers reported the detection of glutathione (GSH) using cationic PT 72 in both colorimetric and fluorescent methods. Chiu and co-workers modified the carboxyl-functionalized ultrabright PFBT CPNs with STA and IgG to perform specific cell imaging. Whitten and co-workers compared the differences regarding the light and dark biocidal activity between cationic PPEs and a cationic PPE alternative.

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    • "CPs possess π-conjugated backbones, large absorption cross sections, good photostability and higher fluorescence relative to small molecules303132. The charged side chains of CPs endow them with sufficient water solubility33. The advantages of CPs mean that they can be used in solid detection platforms to detect multiple targets in a single experiment3435. "
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    ABSTRACT: A new cationic conjugated polymer was designed and synthesized to optically discriminate coenzyme A(CoA) among structurally similar biomolecules, ATP, ADP and AMP. The analyte-induced aggregation of the conjugated polymer by π-stacking between their main chains leads to the fluorescence quenching. Except for the similar adenosine and phosphate moieties as those in ATP, ADP and AMP, the CoA molecule also includes a long side chain that is favorable for hydrophobic interactions. Thus, CoA can form a complex with oppositely charged conjugated polymer by cooperative electrostatic and hydrophobic interactions, whereas ATP, ADP and AMP form the complexes with oppositely charged conjugated polymer mainly by electrostatic interactions. The increase of the ion strength of the assay solution screens the electrostatic attractions, and the remaining hydrophobic interactions dominate the formation of PFP-PTF/CoA complex. At this case, the quenching efficiency of PFP-PTF by CoA is much higher than that by ATP, ADP and AMP, which impart the PFP-PTF to sense CoA from these interferencing species.
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