Microsomal elongases are proteins catalyzing the condensation of malonyl-CoA with acyl-CoA chains, the first and rate-limiting step in microsomal fatty acid elongation. Here we report the measurement of elongase activity of a microsomal enriched fraction from the marine microalga Pavlova lutheri (P. lutheri). By directly monitoring the production of C2 elongated acyl-CoA from a range of saturated and monounsaturated acyl-CoA substrates, we found that saturated 16:0-CoA is the preferred substrate for this elongase complex. Analysis of an EST database prepared from the exponential stage of growth of P. lutheri revealed the most abundant identifiable enzyme as a cDNA, Plelo1, encoding a protein similar to the plant β-ketoacyl-coenzyme A synthases (KCS, also known as elongases). Plelo1 is a single copy gene in the algal genome and gene expression analysis showed it to be highly expressed during the exponential phase of growth. It is suggested that microsomal elongation of 16:0-CoA represents a key intermediate step in the biosynthesis of the health beneficial very long chain polyunsaturated fatty acids eicosapentaenoic (20:5n3) and docosahexaenoic (22:6n3) acids.
[Show abstract][Hide abstract] ABSTRACT: The paper presents a cost effective and increased performance
position servo system using the TMS320F240 digital signal processor
(DSP) produced by Texas Instruments as microprocessor and brushless
direct current motor (BLDCM) as executor. In order to make up for the
drawback of conventional PID controls, the fuzzy PID is employed. The
result of simulations and experiments has confirmed that the whole
system is simple and reliable; the robustness of the system is improved
by using fuzzy PID
Electrical Machines and Systems, 2001. ICEMS 2001. Proceedings of the Fifth International Conference on; 09/2001
[Show abstract][Hide abstract] ABSTRACT: Microalgae are now the focus of intensive research due to their potential as a renewable feedstock for biodiesel. This research requires a thorough understanding of the biochemistry and genetics of these organisms' lipid-biosynthesis pathways. Genes encoding lipid-biosynthesis enzymes can now be identified in the genomes of various eukaryotic microalgae. However, an examination of the predicted proteins at the biochemical and molecular levels is mandatory to verify their function. The essential molecular and genetic tools are now available for a comprehensive characterization of genes coding for enzymes of the lipid-biosynthesis pathways in some algal species. This review mainly summarizes the novel information emerging from recently obtained algal gene identification.
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