Acyl-CoA elongase activity and gene from the marine microalga Pavlova lutheri (Haptophyceae)

UMR 7139 CNRS-GOEMAR-UPMC Station Biologique BP 74 F-29682 Roscoff cedex France
Journal of Applied Phycology (Impact Factor: 2.49). 02/2005; 17(2):111-118. DOI: 10.1007/s10811-005-8119-2

ABSTRACT Microsomal elongases are proteins catalyzing the condensation of malonyl-CoA with acyl-CoA chains, the first and rate-limiting step in microsomal fatty acid elongation. Here we report the measurement of elongase activity of a microsomal enriched fraction from the marine microalga Pavlova lutheri (P. lutheri). By directly monitoring the production of C2 elongated acyl-CoA from a range of saturated and monounsaturated acyl-CoA substrates, we found that saturated 16:0-CoA is the preferred substrate for this elongase complex. Analysis of an EST database prepared from the exponential stage of growth of P. lutheri revealed the most abundant identifiable enzyme as a cDNA, Plelo1, encoding a protein similar to the plant β-ketoacyl-coenzyme A synthases (KCS, also known as elongases). Plelo1 is a single copy gene in the algal genome and gene expression analysis showed it to be highly expressed during the exponential phase of growth. It is suggested that microsomal elongation of 16:0-CoA represents a key intermediate step in the biosynthesis of the health beneficial very long chain polyunsaturated fatty acids eicosapentaenoic (20:5n3) and docosahexaenoic (22:6n3) acids.

  • [Show abstract] [Hide abstract]
    ABSTRACT: The paper presents a cost effective and increased performance position servo system using the TMS320F240 digital signal processor (DSP) produced by Texas Instruments as microprocessor and brushless direct current motor (BLDCM) as executor. In order to make up for the drawback of conventional PID controls, the fuzzy PID is employed. The result of simulations and experiments has confirmed that the whole system is simple and reliable; the robustness of the system is improved by using fuzzy PID
    Electrical Machines and Systems, 2001. ICEMS 2001. Proceedings of the Fifth International Conference on; 09/2001
  • [Show abstract] [Hide abstract]
    ABSTRACT: Microalgae are now the focus of intensive research due to their potential as a renewable feedstock for biodiesel. This research requires a thorough understanding of the biochemistry and genetics of these organisms' lipid-biosynthesis pathways. Genes encoding lipid-biosynthesis enzymes can now be identified in the genomes of various eukaryotic microalgae. However, an examination of the predicted proteins at the biochemical and molecular levels is mandatory to verify their function. The essential molecular and genetic tools are now available for a comprehensive characterization of genes coding for enzymes of the lipid-biosynthesis pathways in some algal species. This review mainly summarizes the novel information emerging from recently obtained algal gene identification.
    Biochimie 01/2011; 93(1):91-100. · 3.14 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Pseudochattonella farcimen (Eikrem, Edvardsen, et Throndsen) is a unicellular alga belonging to the Dictyochophyceae (Heterokonta). It forms recurring blooms in Scandinavian coastal waters, and has been associated to fish mortality. Here we report the sequencing and analysis of 10,368 expressed sequence tags (ESTs) corresponding to 8,149 unique gene models from this species. Compared to EST libraries from other heterokonts, P. farcimen contains a high number of genes with functions related to cell communication and signaling. We found several genes encoding proteins related to fatty acid metabolism, including eight fatty acid desaturases and two phospholipase A2 genes. Three desaturases are highly similar to Δ4-desaturases from haptophytes. P. farcimen also possesses three putative polyketide synthases (PKSs), belonging to two different families. Some of these genes may have been acquired via horizontal gene transfer by a common ancestor of brown algae and dictyochophytes, together with genes involved in mannitol metabolism, which are also present in P. farcimen. Our findings may explain the unusual fatty acid profile previously observed in P. farcimen, and are discussed from an evolutionary perspective and in relation to the ichthyotoxicity of this alga.
    Protist 08/2011; 163(1):143-61. · 3.56 Impact Factor