Factors affecting in vitro propagation of Momordica balsamina: A medicinal and nutritional climber

Defense Research and Development Establishment (DRDE), Ministry of Defence, Govt. of India, Jhansi Road, Gwalior, 474002 India
Physiology and Molecular Biology of Plants 04/2011; 17(2):193-197. DOI: 10.1007/s12298-011-0052-z


A protocol was developed for multiple shoot induction in a climber with highly medicinal and nutritional values i.e. Momordica balsamina (Cucurbitaceae). Effects of various factors on micropropagation were also studied. Addition of BAP at 1.0mg/l in MS medium
stimulated proliferation of shoots with 6–8 coefficients from the axillary buds. Low concentration of activated charcoal (0.2 %)
promoted shoot elongation and reduced callus formation. The combination of 1.0mg/l BAP and 1.0mg/l KN promoted shoot elongation
and proliferation. NAA proved to be more effective in inducing higher number of roots per shoot than IBA. The plantlets raised
in vitro were acclimatized in green house and successfully transplanted to natural condition with 85 % survival. The present
investigation showed that direct regeneration of M. balsamina offer a good opportunity to use micropropagation technique as a complementary tool for breeding and other applications.

Momordica balsamina
–In vitro propagation–Climber–Momordin

Download full-text


Available from: Prakash S Bisen, Oct 05, 2015
65 Reads
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Momordica balsamina, African pumpkin (Cucurbitaceae), is a tendril-bearing, wild climber containing wide spectrum of medicinal and nutritional values and has been used as a traditional folk medicine in many countries. The leaves, fruits, seeds, and bark of the plant contains resins, alkaloids, flavonoids, glycosides, steroids, terpenes, cardiac glycoside, saponins having various medicinal importance viz. anti-HIV, anti-plasmodial, shigellocidal, anti-diarrheal, anti-septic, anti-bacterial, anti-viral, anti-inflammatory, anti-microbial, hypoglycemic, antioxidant, analgesic and hepatoprotective properties. The therapeutic agent 'Momordin' is capable of inhibiting the growth of HIV and other viruses. The leaves are also important source of nutrients having 17 amino acids with adequate mineral composition like potassium, magnesium, phosphorus, calcium, sodium, zinc, manganese and iron. It also helps to combat the problem of micronutrient deficiencies in soil and high value of protein and fat with low fibre content. High potassium content is a good source for the management of hypertension and other cardiovascular conditions. This plant is being promoted as a protein supplement for cereal-based diets in poor rural communities. The commercial exploitation of this plant for biopharmaceuticals and neutraceuticals are some of the prospective future potential of this wild herb. This review discusses the potential of medicinal and nutritional importance of this wild herb for health care management.
    Current pharmaceutical biotechnology 09/2009; 10(7):667-82. · 2.51 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Ten Nigerian medicinal plants used traditionally for the treatment of several ailments of both microbial and non-microbial origins were tested on multi-drug resistant S. typhi (MDR) strains of which six of them were active. The results revealed that both the aqueous and ethanol extracts of Terminalia avicennioides, Momordica balsamina, Combretum paniculatum and Trema guineensis were effective on the MDR-S. typhi strains with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values ranging from 9.60 to 14 mcg/ml and 24 to 33 mcg/ml, respectively. Whereas, only the aqueous extracts of Morinda lucida and Ocimum gratissimum were found to be active against this pathogen with MIC and MBC values of 9.60 and 24 mcg/ml for M. lucida, 40 and 55 mcg/ml for O. gratissimum, respectively. There was no statistical significant difference (P > 0.05) between the activity of each plant extract and the decoctions prepared from them. All the six active plants showed positive reactions to alkaloids, tannins, flavonoids and anthraquinones but in variable degrees. All but M. balsamina, indicated the presence of saponin.
    Journal of Herbal Pharmacotherapy 08/2005; 5(1):45-60. DOI:10.1300/J157v05n01_06
  • Source
    Nucleic Acids Research 10/1992; 20(17):4662. DOI:10.1093/nar/20.17.4662 · 9.11 Impact Factor
Show more