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Factors affecting in vitro propagation of Momordica balsamina: a medicinal and nutritional climber

Research and Development Centre, Bisen Biotech and Biopharma Pvt. Ltd, Biotech Research Park, M-7, Laxmipuram, Transport Nagar, Gwalior, 474010 MP, India; Defense Research and Development Establishment (DRDE), Ministry of Defence, Govt. of India, Jhansi Road, Gwalior, 474002 India
Physiology and Molecular Biology of Plants 04/2011; 17(2):193-197. DOI: 10.1007/s12298-011-0052-z

ABSTRACT A protocol was developed for multiple shoot induction in a climber with highly medicinal and nutritional values i.e. Momordica balsamina (Cucurbitaceae). Effects of various factors on micropropagation were also studied. Addition of BAP at 1.0mg/l in MS medium
stimulated proliferation of shoots with 6–8 coefficients from the axillary buds. Low concentration of activated charcoal (0.2 %)
promoted shoot elongation and reduced callus formation. The combination of 1.0mg/l BAP and 1.0mg/l KN promoted shoot elongation
and proliferation. NAA proved to be more effective in inducing higher number of roots per shoot than IBA. The plantlets raised
in vitro were acclimatized in green house and successfully transplanted to natural condition with 85 % survival. The present
investigation showed that direct regeneration of M. balsamina offer a good opportunity to use micropropagation technique as a complementary tool for breeding and other applications.

Keywords
Momordica balsamina
–In vitro propagation–Climber–Momordin

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