Article

Immunohistochemical analysis of cAMP response element-binding protein in mouse testis during postnatal development and spermatogenesis

Histochemie (impact factor: 2.59). 04/2012; 131(4):501-507. DOI:10.1007/s00418-009-0554-8 pp.501-507

ABSTRACT Basal activity and cellular localization of cAMP response element-binding protein (CREB) was examined in mouse testis during
postnatal development and spermatogenesis. Testes of ICR mice sampled on postnatal day (PND) 3, 7, 14, 21, 28, 35, 42, and
49 were analyzed using Western blotting. Basal CREB activity was significantly higher in early phase (PND 3–7) developing
testes than in intermediate- and late-phase developing (PND 14–42) and adult testes (PND 49). Furthermore, immunohistochemical
analysis demonstrated the change of CREB phosphorylation in various testicular cell types during postnatal development. In
particular, CREB phosphorylation in seminiferous tubules of the adult testis varied according to the spermatogenic cycle,
while phosphorylation was evident in spermatogonia during all stages. Phosphorylation was moderate in pachytene spermatocytes
of stages I–III and intense in round and elongate spermatids of spermiogenesis in stages XII–IX. These results suggest that
CREB plays an important role in cell proliferation and differentiation in the early phase of postnatal development and spermatogenesis
of mouse testis.

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Keywords

adult testes
 
adult testis varied
 
Basal activity
 
Basal CREB activity
 
cAMP response element-binding protein
 
cell proliferation
 
cellular localization
 
CREB phosphorylation
 
elongate spermatids
 
ICR mice sampled
 
late-phase
 
pachytene spermatocytes
 
particular
 
Phosphorylation
 
PND
 
postnatal development
 
seminiferous tubules
 
spermatogenic cycle
 
Testes
 
various testicular cell types