Rat serum electrolytes, lipid profile and cardiovascular activity onNauclea latifolia leaf extract administration

Indian Journal of Clinical Biochemistry 07/2005; 20(2):29-34. DOI: 10.1007/BF02867397


Aqueous extract of the leaf and root ofNauclea latifolia Sm. (Rubiaceae) is used in Nigerian folk medicine for the treatment of hypertension. This work is carried out to investigate
the effect ofNauclea latifolia leaf extract on lipid profile and cardiovascular activity of rats. Normal and 10% coconut oil fed rats were treated with
the water-soluble fraction of the ethanol extract ofNauclea latifolia leaf for 2 weeks. Forty-eight mature male albino rats of the Wistar strain were divided into two experiments of four groups,
each group having 6 animals. Experiment I animals were treated with the water-soluble fraction of the ethanol extract whilst
experiment II animals were fed 10% coconut oil meal before treatment with the water-soluble fraction of the ethanol extract.
A single oral dose ofNauclea latifolia was 170, 340 and 510 mg/kg body wt/day of the extracts respectively for 2 wks. There was no significant change in the lipid
profile of the experimental animals as compared with the controls. There was about 40% relaxation on contracted thoracic aorta
that was pre-contracted with 2 μM phenylephrine. The viability of the tissue was tested against 10 μM of acetylcholine. There
was no significant (P>0.05) change in Na+ concentration in the serum. However, the K+ concentration in the serum of the experimental animals showed a significant increase. The study shows that ethanol extract
ofNauclea latifolia has vasodilator action on the aorta and that lipid profiles of experimental rats were not affected. Furthermore, the increase
in the K+ may be contributing to the vasodilator effect ofNauclea latifolia.

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    • "The preparation of the 10% virgin coconut oil meal was done on regular demand [9] "
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    ABSTRACT: Virgin coconut oil (VCO) is a saturated fat with promising antidiabetic properties but its ameliorative effect on lipid profiles in diabetics is rarely reported. Therefore, in this study, a total of fifteen (15) male rats weighing 200–250 g were divided into 3 experimental groups (.. = 5).Group I (control) andGroup II (diabetic control group) were fed a normal rat chowwhile Group III (diabetic test group) was fed a 10% VCO diet for 3 weeks. Group II and Group III were made diabetic by intraperitoneal injection of 150mg/kg of alloxan. After 72 hours of injection, blood glucose was tested to confirm diabetes mellitus. After 3 weeks, the animals were sacrificed to collect blood samples for lipid profile analysis.Theresults showed a significant increase in concentrations of triglyceride, total cholesterol, low density lipoprotein, and very low density lipoprotein and decrease in concentration of high density lipoprotein in Group II when compared to Group I. Also, the concentrations of triglyceride, total cholesterol, low density lipoprotein, and very low density lipoprotein except high density lipoprotein significantly reduced in Group III when compared to Group II (.. < 0.01, 0.001). VCO consumption can be claimed to ameliorate lipid levels in diabetes mellitus.
    09/2014; 2014(Article ID 256236,):5 Pages. DOI:10.1155/2014/256236
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    • "It has also been used to unravel blood -related functions of chemical compounds including plant extracts (Yakubu et al., 2007). The effects of medicinal plant products on hematological parameters of experimental animals have been performed by several workers (Akpanabiatu et al., 2005; Aboderin and Oyetayo, 2006). These studies have been particularly helpful in assessing the safe use or otherwise of such compounds and plant extracts. "
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    ABSTRACT: The purpose of this study was to identify the sub-acute toxic effects of Khat (Catha edulis) on hemopoiesis and hematological indices of white albino rats. Two groups, each of 10 rats, were used. In the experimental group, a hydro-ethanol extract of C. edulis was administered orally to rats, daily, in single doses of 500 mg/kg body weight, for for weeks. The control group received equivalent amounts of normal saline. Our results show, for the first time, that oral administration of C. edulis hydro-ethanol extract caused significant derangement in hemopoiesis and in gross hematological indices in rats, characterized by macrocytic anemia and leucopenia. Our data show statistically significant decreases in total leukocytes count (TLC) in which, hemoglobin concentration (Hb. conc.), packed cell volume (PCV), and red cell count (RCC), accompanied by significant increases in mean cell volume (MCV), red blood cell distribution width (RDW) and platelets count with no change in mean hemoglobin concentration (MHC). In peripheral blood smears (PBS) of treated rats, there were evidences of dyserythropoiesis- impaired hemoglobinization, macrocytosis, poikilocytosis and anisocytosis, and dysgranulopoiesis- giant forms, hypersegmented neutrophils and bizarre nuclear shapes. In conclusion, our results indicate that oral administration of a hydro-ethanol extract of C. edulis adversely affected blood cell formation and induced macrocytic anemia and leukopenia in rats. However, the exact mechanisms of these hematological changes produced by Khat are still in need for further studies.
    AFRICAN JOURNAL OF BIOTECHNOLOGY 01/2014; 13(2):349. DOI:10.5897/AJB2013.13373 · 0.57 Impact Factor
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    • "Nauclea latifolia (Rubiaceae) is a shrub commonly called pincushion tree (English), mbom-ibong (Ibibio) and tabashiya (Hausa), ubuluinu (Igbo) and scille maritime (French) have been reported to posses hypoglycemic property[3]. Preliminary phytochemical screening by [2] showed that it contains alkaloids, saponins and polyphenols. This study will help to unravel the effect of the ethanol extract and its fractions on the lipid profile of diabetic rats as well as establishing the fraction with the highest hypolipidaemic property. "
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    ABSTRACT: The ethanol extract and its fractions were evaluated in the study to unravel their effects on the lipid profile of diabetic rats after daily administration for a period of two weeks. The analysis of the results showed that there were significant decreases (p < 0.05) in the HDL-cholesterol and total cholesterol levels in all the treatment groups. Insignificant decreases (p > 0.05) in TG levels were recorded in all the treatment groups where as VLDL-C levels significantly decreased (p < 0.05) in the groups treated with glibenclamide, methanol fraction (100 mg/kg) as well as butanol and ethyl acetate fractions. The result of the phytochemical screening showed heavy presence of saponins, flavonoids, phenols, terpenoids, cardiac glycosides and carbohydrates in ethyl acetate, butanol and methanol fractions which may be the reason for the hypolipidaemic property of the above fractions and suggest its potency in arresting oxidative stress in diabetic rats. Also, the n-hexane and ethyl acetate fractions as well as ethanol extract gave hope in arresting hyperlipidemia resulting from diabetes mellitus in diabetic rats.
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