Microfluidic techniques for dynamic single-cell analysis

Microchimica Acta (Impact Factor: 3.74). 03/2010; 168(3):177-195. DOI: 10.1007/s00604-010-0296-2


Dynamic single-cell analysis is a very important and frontier research field of single-cell analysis. Microfluidic techniques
have become new and effective tools for precise, high-throughput, automatic analysis of single-cell dynamic process. This
review aims to give an overview of dynamic single-cell analysis methods based on microfluidic platforms, with emphasis on
the recent developments of microfluidic devices and its application to real-time dynamic monitoring of the signal molecules
release from single living cell with temporal and spatial resolution, dynamic gene expression in single cells, the cell death
dynamic events at the level of a single cell, and direct cell—cell communication between individual cell pairs.

KeywordsDynamic single-cell analysis-Microfluidic techniques-Real-time dynamic monitoring

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