Maternal high-fat diet is associated with altered pancreatic remodelling in mice offspring.

Abstract

PURPOSE: To investigate whether a maternal high-fat diet (HF) during pregnancy and/or suckling periods predisposes adult C57BL/6 mice offspring to morphological pancreatic modifications. METHODS: Male pups were divided into 5 groups: SC (standard chow)-from dams fed SC during gestation and lactation, maintaining an SC diet from postweaning to adulthood; G-from dams fed HF diets during gestation; L-from dams fed HF diets during lactation; GL-from dams fed HF diets during gestation and lactation; and GL/HF-from dams fed HF diets during gestation and lactation, maintaining an HF diet from postweaning to adulthood. We analysed body mass (BM), plasma insulin, pancreas and adipose tissue structures. RESULTS: During the entire experiment, the SC group had the lowest BM. However, GL/HF offspring were heavier than the other groups. This weight gain was also accompanied by adipocyte hypertrophy. At 3 months, G offspring showed an increased insulin levels and impairment in carbohydrates metabolism. Furthermore, pancreatic islets were hypertrophied in G, GL and GL/HF offspring in comparison with SC offspring. CONCLUSION: HF diet administration during the gestation period is more harmful than during the lactation period, exerting deleterious effects on pancreatic morphology in addition to larger fat deposits in adult mice offspring.