Behavioral barcoding in the cloud: Embracing data-intensive digital phenotyping in neuropharmacology

Cardiovascular Research Center and Division of Cardiology, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Charlestown, MA 02129, USA.
Trends in Biotechnology (Impact Factor: 10.04). 05/2012; 30(8):421-5. DOI: 10.1016/j.tibtech.2012.05.001
Source: PubMed

ABSTRACT For decades, studying the behavioral effects of individual drugs and genetic mutations has been at the heart of efforts to understand and treat nervous system disorders. High-throughput technologies adapted from other disciplines (e.g., high-throughput chemical screening, genomics) are changing the scale of data acquisition in behavioral neuroscience. Massive behavioral datasets are beginning to emerge, particularly from zebrafish labs, where behavioral assays can be performed rapidly and reproducibly in 96-well, high-throughput format. Mining these datasets and making comparisons across different assays are major challenges for the field. Here, we review behavioral barcoding, a process by which complex behavioral assays are reduced to a string of numeric features, facilitating analysis and comparison within and across datasets.

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    • "Zebrafish have emerged as versatile models of vertebrate biology, due to their amenability to genetic and pharmacological manipulations, optical transparency during embryogenesis and larval development, and facile and economical husbandry [1]–[4]. They have been used extensively to investigate the molecular and cellular mechanisms that contribute to tissue patterning [5] and more recently have contributed to our understanding of tissue regeneration, tumorigenesis, metabolism, infectious disease, and behavior [6]–[11]. As the importance of teleost models in biomedical research continues to grow, transgenic lines that can provide real-time indicators of specific biological events will be increasingly valuable. "
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    PLoS ONE 07/2014; 9(7):e103661. DOI:10.1371/journal.pone.0103661 · 3.23 Impact Factor
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    • "This may, in turn, decrease the risk for relapse and increase tobacco abstinence rates. With the advent of the validated behavioral screen described here, primed with initial drug evaluation data, the development of a high-throughput screening method for pharmacotherapeutic modifiers of nicotine and ethanol response is now warranted, and a number of studies have shown measuring larval locomotion is amenable to high throughput approaches [69], [81], [82]. Moreover, exploiting this model to evaluate medications approved for human use by the FDA enables clinicians to study these medications in clinical trials without further preclinical safety testing. "
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    • "Zebrafish are popular as a vertebrate model with which to perform compound screens [3,4] and increasingly used to model human neurological disease processes, such as epilepsy and neurodegeneration [5,6]. Therefore, it is important to understand whether and when the zebrafish BBB forms and compare its characteristics and function with that of mammals. "
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