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Phylogenetic analysis of benign Theileria species based on major piroplasm surface protein (MPSP) genes from ticks of grazing cattle in Korea

Parasitology and Insect Disease Research Laboratory, Animal, Plant and Fisheries Quarantine and Inspection Agency, 480 Anyang 6 dong, Anyang City 420-480, Republic of Korea.
Veterinary Parasitology (Impact Factor: 2.55). 05/2012; 189(2-4):145-52. DOI: 10.1016/j.vetpar.2012.04.038
Source: PubMed

ABSTRACT Complete major piroplasm surface protein (MPSP) gene sequences of benign Theileria parasites were isolated from ticks of grazing cattle in Korea. A total of 556 tick samples were collected in five provinces: Chungbuk, Jeonbuk, Jeonnam, Gyeongbuk, and Jeju during 2010-2011. Fifteen samples from Chungbuk and Jeonnam were positive for the Theileria MPSP gene by PCR amplification using a specific primer set. A phylogenetic tree was constructed with the amplified gene sequences and 26 additional sequences published in GenBank. The benign Theileria parasites were classified into eight types, those isolated from Korean cattle ticks belonged to Types 1 (Ikeda), 2 (Chitose), 4, and 8. Types 2 and 4 were the most common types, with the rate of 40%, followed by Types 1 and 8 (with the rate of 13% and 7%, respectively). Nucleotide sequence identities of 23 theilerial MPSP sequences (15 MPSP gene sequences amplified and 8 sequences published) ranged from 67.3 to 99.8%. Multiple alignments of the deduced amino acid sequences also showed that each type was characterized by specific amino acids: 7 for Type 1, 9 for Type 2, 4 for Type 4, and 3 for Type 8.

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    • "Other investigators showed substantially less sequence variation in the widely-used mpsp gene within genotypes (buffeli: 0–5.9%; chitose: 0–2%; ikeda: 0–1.7%) compared with differences among them (11.9–19.4%) (Kim et al., 1998; Kawazu et al., 1999; Gubbels et al., 2000; Zakimi et al., 2006; Ota et al., 2009; Altangerel et al., 2011; Islam et al., 2011; Kamau et al., 2011a; Khukhuu et al., 2011; Cufos et al., 2012; Kang et al., 2012; Perera et al., 2013; Sivakumar et al., 2013). Thus, although inter-genotypic differences determined in the present study are lower than those obtained in previous studies for other nuclear genes, they are substantially higher than the degree of intra-genotypic sequence variability, which indicates that the p23 gene region employed here is useful for the identification of T. orientalis genotypes in Victoria, Australia, and is likely applicable in other parts of the world. "
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    • "Each MPSP sequence determined was compared with reference sequences available for members of the T. orientalis complex in current databases (15 January 2013), and sequence identities (in %) calculated by pairwise comparison . Subsequently, all MPSP sequences were aligned with reference sequences of T. orientalis, and of T. annulata and T. parva (outgroups) (Altangerel et al. (2011), Govaerts et al. (1998), Kang et al. (2012), Kawazu et al. (1992, 1999), Kim et al. (1998), Khukhuu et al. (2011), Matsuba et al. (1993), Sarataphan et al. (2003), Shiels et al. (1995), Sivakumar et al. (2012); see Supplementary Table 1) over a consensus length of 309 bp. The phylogenetic analysis of sequence data was conducted using Bayesian inference (BI) and Neighbour-Joining methods (NJ) (cf. "
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