Genetic variation in DNA repair gene XRCC7 (G6721T) and susceptibility to breast cancer

Department of Biology, College of Sciences, Shiraz University, Shiraz 71454, Iran.
Gene (Impact Factor: 2.08). 05/2012; 505(1):195-7. DOI: 10.1016/j.gene.2012.04.065
Source: PubMed

ABSTRACT The human XRCC7 is a DNA double-strand break (DSBs) repair gene, involved in non-homologous end joining (NHEJ). It is speculated that DNA DSBs repair have an important role during development of breast cancer. The human XRCC7 is a NHEJ DSBs repair gene. Genetic variation G6721T of XRCC7 (rs7003908) is located in the intron 8 of the gene. This polymorphism may regulate splicing and cause mRNA instability. In the present study, we specifically investigated whether common G6721T genetic variant of XRCC7 was associated with an altered risk of breast cancer. The present study included 362 females with breast cancer. Age frequency-matched controls (362 persons) were randomly selected from the healthy female blood donors, according to the age distribution of the cases. Using RFLP-PCR based method, the polymorphism of XRCC7 was determined. The TG (OR=1.20, 95% CI: 0.83-1.74, P=0.320) and TT (OR=1.01, 95% CI: 0.67-1.53, P=0.933) genotypes had no significant effect on risk of breast cancer, in comparison with the GG genotype. Our present findings indicate that the TT and TG genotypes were not associated with an altered breast cancer risk.

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    ABSTRACT: Previous investigations indicated that histamine receptor H4 (HRH4) played important roles in many aspects of breast cancer pathogenesis, and that the polymorphisms of HRH4 gene may result in expression and functional changes of HRH4 proteins. However, the relationship between polymorphisms of HRH4 and breast cancer risk and malignant degree is unclear. In the present study, we conducted a case-control investigation among 185 Chinese Han breast cancer patients and 199 ethnicity-matched health controls. Four tag-SNPs (i.e. rs623590, rs16940762, rs11662595 and rs1421125) of HRH4 were genotyped and association analysis was performed. Odds ratios (ORs) with 95% confidence intervals (CI) were used to assess the association. We found that the T allele of rs623590 had a decreased risk of breast cancer (adjusted OR, 0.667; 95% CI, 0.486-0.913; P=0.012) while the A allele of rs1421125 had an increased risk (adjusted OR, 1.653; 95% CI, 1.139-2.397; P=0.008). Further haplotype analysis showed that the CAA haplotype of rs623590-rs11662595-rs1421125 was more frequent among patients with breast cancer (adjusted OR, 1.856; 95% CI, 1.236-2.787; P=0.003). Additionally, polymorphisms of rs623590 and rs11662595 were also correlated with clinical stages, lymph node involvement, and HER2 status. These findings indicated that the variants of rs623590, rs11662595 and rs1421125 genotypes of HRH4 gene were significantly associated with the risk and malignant degree of breast cancer in Chinese Han populations, which may provide us novel insight into the pathogenesis of breast cancer although further studies with larger participants worldwide are still needed for conclusion validation.
    Gene 02/2013; DOI:10.1016/j.gene.2013.02.020 · 2.08 Impact Factor
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    ABSTRACT: Background:Prostate cancer (PCa) gene expression marker 1 (PCGEM1), a long noncoding RNA, has drawn increasing attention for its important role in PCa. However, the association between genetic variations in the PCGEM1 gene and risk of PCa has not been investigated yet.Methods:We investigated the effect of two tagging single-nucleotide polymorphism (tSNPs; rs6434568 and rs16834898) in PCGEM1 gene on PCa risk in the Chinese men. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the association.Results:We found a significantly decreased risk of PCa for rs6434568 AC and AC/AA genotype (adjusted OR=0.76, 95% CI=0.60-0.97 for AC; adjusted OR=0.76, 95% CI=0.61-0.96 for AC/AA), as well as rs16834898 AC and AC/CC genotype (adjusted OR=0.76, 95% CI=0.59-0.97 for AC; adjusted OR=0.79, 95% CI=0.62-0.99 for AC/CC), compared with the CC and AA genotypes, respectively. When we evaluated these two tSNPs together based on the risk alleles (that is, rs6434568 C and rs16834898 A), we found that the combined genotypes with four risk alleles were associated with an increased risk of PCa compared with those carrying 0-3 risk alleles (1.53, 1.19-1.97), and this increased risk was more pronounced among subjects of70 years (1.80, 1.24-2.62), Gleason score7 (1.68, 1.28-2.22) and PSA level20 (1.64, 1.24-2.18).Conclusions:Our results indicated that PCGEM1 polymorphisms may contribute to PCa risk in Chinese men. Additional functional analyses are required to detect the detailed mechanism underlying the observed association.Prostate Cancer and Prostatic Disease advance online publication, 5 March 2013; doi:10.1038/pcan.2013.6.
    Prostate cancer and prostatic diseases 03/2013; DOI:10.1038/pcan.2013.6 · 2.83 Impact Factor
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    ABSTRACT: The association between the rs7003908 (T>G) polymorphism in the XRCC7 gene and the risk of cancers had been widely studied; however, the results were inconsistent. The objective of the current study was to investigate the association between the rs7003908 polymorphism in the XRCC7 gene and the risk of cancers by meta-analysis. We searched PubMed, EMbase, CNKI and Wanfang databases; the last search was performed on January 10th, 2014. Statistical analysis was performed using the Revman4.2 and STATA10.0 softwares. A total of 3,684 cancer cases and 5,232 controls from 11 case-control studies were included for data analysis. In the dominant model analysis, the results suggested a lack of association between the polymorphism and the risk of cancers: OR 1.01, 95 % CI 0.83-1.16, P = 0.70. In the subgroup analysis by ethnicity, no significant association was found either for Asians or Caucasians. In the subgroup analysis by cancer types, significant association was found for prostate cancer, but not for bladder cancer, breast cancer and glioma. In summary, the current meta-analysis confirmed that the rs7003908 polymorphism in the XRCC7 gene might be a risk factor for prostate cancer. In the future, more studies are needed to validate these results.
    Molecular Biology Reports 02/2014; 41(6). DOI:10.1007/s11033-014-3220-8 · 1.96 Impact Factor