Retrospective study of cytologic features of well-differentiated hepatocellular carcinoma in dogs

San Marco Private Veterinary Laboratory, Padua, Italy.
Veterinary Clinical Pathology (Impact Factor: 1.27). 05/2012; 41(3):382-90. DOI: 10.1111/j.1939-165X.2012.00436.x
Source: PubMed


Cytologic diagnosis of hepatocellular carcinoma is possible when hepatocytes have prominent criteria of malignancy, but is problematic when hepatocytes have a relatively normal morphologic appearance.
The aim of this study was to determine the cytologic features of histologically confirmed well-differentiated hepatocellular carcinomas (WD-HCC) in dogs to develop reasonable criteria for an accurate diagnosis when obvious criteria of malignancy in hepatocytes are lacking.
Slides of ultrasound-guided fine-needle aspirates of 15 histologically confirmed WD-HCC in dogs were evaluated retrospectively using a scoring system (0-3) established for 33 cytologic features. For comparison, 15 cytologic samples of non-nodular non-neoplastic liver were evaluated using the same criteria. Statistical analysis included multiple hypothesis testing using the Benjamini-Hochberg method or false discovery rate control to correct for multiple comparisons.
The most significant and useful cytologic features in the diagnosis of canine WD-HCC were dissociation of hepatocytes, acinar or palisading arrangements of neoplastic cells, and the presence of naked nuclei and capillaries, together with mild anisocytosis, anisokaryosis, multinuclearity, and increased N:C ratios.
Cytologic features of canine WD-HCC were determined. Further studies are warranted to compare these cytologic criteria among WD-HCC, hepatomas, and hyperplastic nodules and to examine co-variance of the 33 cytologic features.

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Available from: Carlo Masserdotti, Jul 02, 2014
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