Retrospective study of cytologic features of well-differentiated hepatocellular carcinoma in dogs.
ABSTRACT Cytologic diagnosis of hepatocellular carcinoma is possible when hepatocytes have prominent criteria of malignancy, but is problematic when hepatocytes have a relatively normal morphologic appearance.
The aim of this study was to determine the cytologic features of histologically confirmed well-differentiated hepatocellular carcinomas (WD-HCC) in dogs to develop reasonable criteria for an accurate diagnosis when obvious criteria of malignancy in hepatocytes are lacking.
Slides of ultrasound-guided fine-needle aspirates of 15 histologically confirmed WD-HCC in dogs were evaluated retrospectively using a scoring system (0-3) established for 33 cytologic features. For comparison, 15 cytologic samples of non-nodular non-neoplastic liver were evaluated using the same criteria. Statistical analysis included multiple hypothesis testing using the Benjamini-Hochberg method or false discovery rate control to correct for multiple comparisons.
The most significant and useful cytologic features in the diagnosis of canine WD-HCC were dissociation of hepatocytes, acinar or palisading arrangements of neoplastic cells, and the presence of naked nuclei and capillaries, together with mild anisocytosis, anisokaryosis, multinuclearity, and increased N:C ratios.
Cytologic features of canine WD-HCC were determined. Further studies are warranted to compare these cytologic criteria among WD-HCC, hepatomas, and hyperplastic nodules and to examine co-variance of the 33 cytologic features.
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Article: Canine hepatocellular carcinoma.[show abstract] [hide abstract]
ABSTRACT: In a study of 110 primary hepatic neoplasms in dogs, 55 hepatocellular carcinomas and two combined hepatocellular and cholangiocarcinomas were diagnosed. These neoplasms were classified into the following 11 groups based on histo-architectural pattern: trabecular, peliod, cobblestone, peritheliomatous, anaplastic, pseudoglandular, pleomorphic, scirrhous, clear cell, solid, and combined hepatocellular and cholangiocarcinoma. The neoplastic hepatocytes varied from almost normal to highly anaplastic spindle cells. Pleomorphic and giant cells were common in some groups, rare or absent in others. Metastasis was found in 61% (35 of 57), in contrast to a much higher percent in man, indicating the possibility of a different pathogenesis in the dog.Veterinary Pathology 08/1981; 18(4):427-38. · 1.93 Impact Factor
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ABSTRACT: Evidence has been accumulating that Mallory body (MB) is a heritable phenotype of hepatocellular carcinoma (HCC) and is closely related to hepatocarcinogenesis in some carcinogen-fed animals. This prompted us to try to evaluate the MB phenomenon in human HCCs, in which its significance remains unclear to date. Whole liver slices were obtained from 28 autopsied livers in which MB-positive HCC was found, and distribution of MBs within the HCC was examined. There were three distribution patterns: clustering, diffuse, and sparse types. The clustering type was the most frequent and was found in 21 of the 28 cases. In this type, there was a formation of groups of the MB-containing cells within the HCC, and the cluster of MB-positive cells was variable in number, size, and shape. There was often a sharp border line between MB-positive clusters and the surrounding MB-negative HCC cell areas where a collision line could occasionally be seen. Some of the MB-containing HCC cells were positive for gamma-glutamyl transpeptidase and alpha-fetoprotein. These data led the authors to speculate that MB-positive HCC cells in human livers are capable of proliferation to form the clusters and that therefore, MBs are expressed as a kind of heritable phenotype in such clusters.Cancer 02/1986; 57(1):81-6. · 5.20 Impact Factor
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ABSTRACT: Well-differentiated hepatocellular carcinoma (HCC) in fine-needle aspiration (FNA) biopsy is characterized by trabeculae three or more cells thick wrapped by peripheral endothelium. The authors encountered another pattern that did not fulfill these classic criteria for malignancy yet was proven to be HCC in clinical follow-up. The objective of this study was to characterize the cytologic features of this pattern with histologic correlations. Over a period of 6.5 years, 14 of 123 cases (11.4%) of HCC from 428 liver aspirates showed the unusual pattern. Their cytologic features were compared with 14 cases of nonneoplastic liver on FNA smears that were processed with Ultrafast Papanicolaou stain, and their histologic features were compared on cell blocks with hematoxylin and eosin stain and reticulin stain. The unusual type of well differentiated HCC in FNA smears was characterized by numerous, small hepatocytes with minimal nuclear atypia but reduced cytoplasm, grouped together in microacini of five or more cells and microtrabeculae one or more cells thick of irregular thickness with no apparent peripheral endothelium. Transgressing capillaries were found when the smears were inspected carefully. In histology nine cases that were the compact type of HCC, two cases that were the microacinar type of HCC, and three cases that were the microtrabecular type of HCC. All FNAs showed deficient reticulin framework. All 14 cases of nonneoplastic liver aspirate were characterized by the presence of large tissue fragments that were resistant to smearing. Well differentiated HCC may present as microtrabeculae of irregular thickness one or more cells thick with inapparent peripheral endothelium or as microacini mimicking neuroendocrine carcinoma.Cancer 03/2004; 102(1):27-33. · 5.20 Impact Factor