Article

Isolation and differentiation potential of fibroblast-like stromal cells derived from human skin.

Department of Medical Biotechnology and Laboratory Science and Research Center for Emerging Viral Infections, Chang Gung University, Tao-Yuan, Taiwan.
Methods in molecular biology (Clifton, N.J.) 01/2012; 879:465-70. DOI:10.1007/978-1-61779-815-3_27 pp.465-70
Source: PubMed

ABSTRACT Isolation of progenitor cell population is the first step in performing stem cell research. Progenitors isolated using different methods usually exhibit different characteristics and varied differentiation potentials. Here, we describe a method for isolation of the multipotent stromal cells from human foreskin tissues. The isolation rate is high and the obtained cells could be easily maintained in DMEM without supplement with specific growth factors.

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Keywords

different methods
 
first step
 
multipotent stromal cells
 
obtained cells
 
specific growth factors
 
varied differentiation potentials