An integrated electrochemical device based on immunochromatographic test strip and enzyme labels for sensitive detection of disease-related biomarkers

Department of Chemistry and Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian Province, China.
Talanta (Impact Factor: 3.55). 05/2012; 94:58-64. DOI: 10.1016/j.talanta.2012.02.046
Source: PubMed


A novel electrochemical biosensing device that integrates an immunochromatographic test strip and a screen-printed electrode (SPE) connected to a portable electrochemical analyzer was presented for rapid, sensitive, and quantitative detection of disease-related biomarker in human blood samples. The principle of the sensor is based on sandwich immunoreactions between a biomarker and a pair of its antibodies on the test strip, followed by highly sensitive square-wave voltammetry (SWV) detection. Horseradish peroxidase (HRP) was used as a signal reporter for electrochemical readout. Hepatitis B surface antigen (HBsAg) was employed as a model protein biomarker to demonstrate the analytical performance of the sensor in this study. Some critical parameters governing the performance of the sensor were investigated in detail. Under optimal conditions, this sensor was capable of detecting a minimum of 0.3 ng mL(-1) (S/N=3) HBsAg with a wide linear concentration range from 1 to 500 ng mL(-1). The sensor was further utilized to detect HBsAg spiked in human plasma with an average recovery of 91.3%. In comparison, a colorimetric immunochromatographic test strip assay (ITSA) was also conducted. The result shows that the SWV detection in the electrochemical sensor is much more sensitive for the quantitative determination of HBsAg than the colorimetric detection, indicating that such a sensor is a promising platform for rapid and sensitive point-of-care testing/screening of disease-related biomarkers in a large population.

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Available from: Yuehe Lin, Apr 21, 2015
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    • "Replacement of conventional electrochemical cells by screen printed electrodes (SPE) connected with portable potentiostats is a main trend in the shift of lab electrochemical equipments to handheld field analyzers [15] [16]. The SPE system can be considered as a disposable electrochemical cell, which reduces the required sample volume, simplifies the apparatus and makes the point-of-care testing easy to handle and cost effective [17] [18] [19]. "
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    ABSTRACT: In this paper, a portable, cost-effective electrochemical assay is presented for rapid, sensitive, and quantitative detection of cytochrome c (cyt c) release. The developed cyt c assay consists of two parts: (i) a miniaturized electrochemical biosensor based on cytochrome c reductase (CcR) functionalized screen printed electrodes (SPE); (ii) a microcontroller based data acquisition unit integrated with potentiostat circuit capable of performing cyclic voltammetry technique for the analysis. The working electrode surface of SPE was integrated with polypyrrole (PPy)-carbon nanotubes (CNT) nanocomposite for an enhanced immobilization of the enzyme, CcR. The acquired biosensor data are processed into digital form by the microcontroller and further transferred to a PC through USB port for analysis. GUI based system implemented here makes the analyzer easy to operate. Under optimal conditions, the electroanalytical behavior of the CcR-CNT-PPy-SPE biosensor linearly responds to the cyt c concentration range from 10 nM to 500 μM with a detection limit of 10 nM and a sensitivity of 0.102 ± 0.005 μA μM−1 cm−2. The performance of the volume miniaturized SPE based biosensor coupled with the portable microcontroller based instrument was further evaluated by applying it for the measurement of mitochondrial cyt c release during cardiomyocytes apoptosis; the results are validated well with the commercial electrochemical analyzer and standard ELISA.
    Sensors and Actuators A Physical 11/2014; DOI: 10.1016/j.sna.2014.10.018. DOI:10.1016/j.sna.2014.10.018 · 1.90 Impact Factor
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    • "Screen printed electrode (SPE) has improved continuously over past few decades, especially in terms of the printing material. With its merits such as the output linearity, low power requirement, quick response, high sensitivity, simple operation, and low cost, SPE has attracted increasing interest in the field of biosensor (Galandová et al. 2009; Ping et al. 2012; Zou et al. 2012). "
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    ABSTRACT: Fe3O4 magnetic nanoparticles (MNPs) and Fe3O4-deposited multi-walled carbon nanotubes (Fe3O4@MWCNTs) were synthesized by ultrasonic co-precipitation method. The surface and structural properties of Fe3O4 MNPs and Fe3O4@MWCNTs were characterized by X-ray diffraction, field emission transmission electron microscopy (FE-TEM), X-ray photoelectron spectroscopy, and dynamic light scattering (DLS). Sedimentation field-flow fractionation (SdFFF) was, for the first time, employed to study the influence of synthesis parameters on size distribution of Fe3O4 MNPs. A reasonable resolution for SdFFF analysis of Fe3O4 MNPs was obtained by a combination of 1,600 RPM, flow rate of 0.3 mL min(-1), and Triton X-100. The results suggest that lower pH and higher reaction temperature tend to yield smaller Fe3O4 MNPs size. The size distribution of Fe3O4 MNPs obtained from SdFFF was compared with those obtained from TEM and DLS. Also the effect of the particle size of Fe3O4 MNPs on electrochemical property of Fe3O4@MWCNTs-treated screen printed electrode (SPE) was studied. Cyclic voltammetry revealed that SPE treated with MWCNTs yields a significantly enhanced signal than that with no treatment. The SPE signal was even further enhanced with addition of Fe3O4 MNPs. For SPE analysis of dopamine, a liner range of 0.005-0.1 mM with a correlation coefficient of 0.986 was observed. Results revealed that (1) SdFFF is a useful tool for size-based separation and characterization of MNPs; (2) Proposed methods for synthesis of Fe3O4 nanoparticles and Fe3O4@MWCNTs are mild and fast (about 30 min); (3) SPE treated with Fe3O4@MWCNTs shows potential applicability for biosensing.
    Journal of Nanoparticle Research 10/2014; 16(10-10):1-12. DOI:10.1007/s11051-014-2679-5 · 2.18 Impact Factor
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    ABSTRACT: Square wave voltammetry (SWV) has been widely used in the development of electrochemical sensors and biosensors in recent years due to its high selectivity and sensitivity. It is of great interest and importance to rapidly and sensitively detect disease-related biomarkers, environmental pollutants (e.g., heavy metals and other chemical contaminants), which are severely detrimental to human and animal life and the environment as a whole. Further, efficacious sensing is required for the detection of food resident contaminants (e.g., bacteria, viruses and parasites) and for the verification of the therapeutic ingredients of dietary supplements. Enzyme kinetics is another interesting domain that employs SWV as an effective analytical tool for the mechanistic study of enzyme reactions. The aim of this paper is to provide a comprehensive review of the electrochemical SWV method and its significant applications in sensing and biosensing spanning various fields such as diagnosis, environmental and food analysis and enzyme kinetics. The development of novel and improved electrode surfaces and nanomaterials introduces the possibility of sensors and biosensors that will exhibit even higher sensitivity, with SWV serving as an ideal methodology for its optimization. Concurrent with a better understanding of electrochemistry and life sciences, sensors and biosensors based on SWV have the potential to serve as next generation point-of-care diagnostic devices, as well as highly sensitive and selective detectors for food/environmental monitoring and enzyme studies.
    Analytical methods 04/2013; 5(9):2158-2173. DOI:10.1039/C3AY40155C · 1.82 Impact Factor
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