Enhanced dispersibility and cellular transmembrane capability of single-wall carbon nanotubes by polycyclic organic compounds as chaperon

Chinese Academy of Sciences Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology of China, Beijing 100190, China.
Nanoscale (Impact Factor: 7.39). 05/2012; 4(13):3983-9. DOI: 10.1039/c2nr30346a
Source: PubMed


The common aggregation of single-wall carbon nanotube (SWCNT) in solution is the critical obstacle to elucidate their unique physico-chemical characteristics and biological properties. Therefore, it is very important to overcome this barrier through manipulation of the weak interaction of small molecules with nanotube surface limited interface. A highly dispersed SWCNT system was achieved by binding with polycyclic organic compounds (POCs) including rhodamine 123, ethidium bromide, fluorescein isothiocyanate and 1-pyrene butyric acid as chaperons, in cooperation with sodium dodecyl sulfate. POCs were believed to penetrate through the interstices of aggregated SWCNTs and bind with individual SWCNTs to form highly dispersed and stable SWCNT-POC-surfactant conjugates in both water and phosphate buffer-serum solution, confirmed by gel electrophoresis, transmission electron microscopy and atomic force microscopy. The possible binding interaction includes π-π stacking with side-wall, electrostatic interactions with defect sites and coating surfactants. Compared to pristine SWCNTs, individual SWCNT-POC conjugates had improved transmembrane passage ability through both endocytosis and diffusion pathways, validated by laser scanning confocal microscopy and micro-Raman mapping techniques. For the applications of SWCNTs in drug delivery, in vitro imaging and other research fields, this novel strategy could provide highly dispersed SWCNTs with better efficiency of drug loading and stability.

Download full-text


Available from: Xue Xue,
  • [Show abstract] [Hide abstract]
    ABSTRACT: The scope of nanotechnology is gaining importance in biology and medicine. Carbon nanotubes (CNTs) have emerged as promising tool due to their unique properties, high specific surface area, and capacity to cross biological barriers. These properties offer a variety of opportunities for applications in nanomedicine, such as diagnosis, disease treatment, imaging, and tissue engineering. Nevertheless, pristine CNTs are insoluble in water and in most organic solvents; thereby functionalization of their surface is necessary to increase biocompatibility. Derivatization of CNTs also gives the possibility to conjugate different biological and bioactive molecules including drugs, proteins, and targeting ligands. This review focuses on the chemical modifications of CNTs that have been developed to impart specific properties for biological and medical purposes. Biomolecules can be covalently grafted or non-covalently adsorbed on the nanotube surface. In addition, the inner core of CNTs can be exploited to encapsulate drugs, nanoparticles, or radioactive elements.
    Advanced drug delivery reviews 07/2013; 65(15). DOI:10.1016/j.addr.2013.07.006 · 15.04 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The different methods that permit to functionalize covalently or non-covalently CNT are described. a large part of this chapter is dedicated to the applications of such modified CNT in nanomedicine, nanoelectronics, compsite materials.
    Applied Surface Chemistry of Nanomaterials, Edited by Mohamed M. Chehimi and Jean Pinson, 10/2013: chapter Carbon N: pages notubes. Surface Modification and applications; Nova., ISBN: 978-1-62808-352-1
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Novel hybrids of fluorescein-labeled poly (ethylene glycol)-modified single-walled carbon nanotubes (SWCNTs) with nucleic acids were prepared. 5'-Pyrene conjugates of oligodeoxyribonucleotides were used to construct the non-covalent hybrids with the pyrene residues acting as anchor groups, immobilizing an oligonucleotide on the SWCNT surface. The hybrid formation characteristics were studied using ζ-potential measurements and adsorption isotherm plots. Transmission electron microscopy (TEM) of the samples stained with contrasting agents proved that the pyrene conjugates of the oligonucleotides were adsorbed onto the surfaces of the functionalized SWCNTs. Based on the MTT assay, the functionalized SWCNTs and their hybrids with oligonucleotides exhibited low toxicity toward HeLa, KB-3-1, and KB-8-5 cells. A TEM study of ultrathin sections of cells treated with SWCNTs revealed that the nanotubes directly interacted with the cellular surface.
    ACS Applied Materials & Interfaces 01/2014; 6(3). DOI:10.1021/am4034729 · 6.72 Impact Factor
Show more