Protective effects of yacon (Smallanthus sonchifolius) intake on experimental colon carcinogenesis
ABSTRACT Yacon (Smallanthus sonchifolius), a tuberous root native to the Andean region of South America, contains high concentration of fructans with potential for colon cancer prevention. This study investigated the potential beneficial of yacon intake on colon carcinogenesis induced by 1,2-dimethylhydrazine (DMH) in male Wistar rats. After 4 weeks of DMH-initiation, groups were fed basal diet (G1 and G6) or basal diet containing dried extract of yacon root at 0.5% (G2), 1.0% (G3 and G5) or a synbiotic formulation (G4) (1.0% yacon plus Lactobacillus casei at 2.5 × 10(10)CFU per g diet) for 13 weeks. At week 20, a significant reduction in number and multiplicity of aberrant crypt foci (ACF) and in number of invasive adenocarcinomas was observed in the groups orally treated with 1.0% yacon (G3) or the synbiotic formulation (G4) (0.05<p<0.001). Tumor multiplicity (noninvasive plus invasive) was significantly lower in the group fed synbiotic formulation (p<0.02). A significant reduction in cell proliferation in colonic crypts and tumors and short chain fatty acids (SCFA) caecal contents was observed in the groups orally treated with 1.0% yacon (G3) or the synbiotic formulation (G4). Therefore, the findings this study indicate that yacon and yacon plus L. casei intake may reduce the development of chemically-induced colon cancer.
- SourceAvailable from: Kátia Sivieri
[Show abstract] [Hide abstract]
- "In the initial phase of tumor progression, groups that ingested AYE (G4) and its association with L. acidophilus CRL 1014 (G5) showed better suppressor response when compared to DMH group (G2) and probiotic (G3), once only 50 and 66.67% of macroscopic tumors in G4 (prebiotic) and G5 (synbiotic) were adenocarcinomas (Fig. 3A). Likewise, de Moura et al. (2012) reported lower proportion of adenocarcinomas induced by DMH in groups receiving a synbiotic formulation (1% yacon root and L. casei at 2.5 × 10 −10 CFU per gram). However, no additional or synergic effects were observed using our synbiotic formulation. "
ABSTRACT: The modifying effects of aqueous yacon extract (AYE) and L. acidophilus CRL 1014 against colon carcinogenesis induced by 1,2-dimethylhydrazine (DMH) in male Wistar rats were investigated. Animals were allocated into five groups: G1: untreated group; G2: DMH-treated group; G3: DMH + L. acidophilus- treated group; G4: DMH + AYE-treated group; G5: DMH + L. acidophilus and AYE-treated group. A significant reduction (p<0.05) in leukocyte DNA damage and in colonic cell proliferation were observed after the first DMH administration in G3 (probiotic), G4 (prebiotic) and G5 (synbiotic) groups. In this moment, a significant increase (p<0.05) in colonic apoptosis was also observed in G3 (probiotic) and G5 (synbiotic) groups. In special, at five months after DMH administrations, a significant reduction (p < 0.05) in ACF development was observed in G3 (probiotic), G4 (prebiotic) and G5 (synbiotic) groups. Incidence of colon tumors was lower at five months in G4 (prebiotic) and G5 (synbiotic) groups but not in eight months after DMH administrations. In conclusion, the findings suggest that the oral treatments have potential effects as a chemopreventive agent against colon carcinogenesis on early stage of tumor development.Food Research International 04/2015; 74. DOI:10.1016/j.foodres.2015.04.034 · 3.05 Impact Factor
[Show abstract] [Hide abstract]
- "The protein expression levels of Ki-67 (i.e., cell proliferation marker), cleaved caspase-3 (i.e., apoptosis marker) and b-catenin and connexin 43 (Cx43) (i.e., tumor progression markers) in the colon tumors were detected immunohistochemically using a polymer system (MACH 4 Universal HRP polymer Detection, Biocare, CA, USA). Briefly, deparaffinated 5 lm colon tumor sections on silanised slides were treated sequentially with 0.01 M citrate buffer (pH 6.0) at 120 °C for 5 min in a Pascal Pressure Chamber (Dako Cytomation Denmark A/S), 3% H 2 O 2 in PBS for 10 min, nonfat milk for 60 min, either mouse anti-Ki-67 (1:100 dilution, Abcam ab16667, Cambridge, MA 02139), rabbit polyclonal cleaved anti-caspase-3 (clone Asp 175, 1:100 dilution, Cell Signalizing Technology, Inc., Danvers, MA, USA), rabbit polyclonal anti-b-catenin (clone ab6302, Abcam, MA, USA) or rabbit polyclonal anti- Cx43 (clone GJA1, Abcam, MA, USA) antibodies overnight at 4 °C, the mouse probe for 30 min at room temperature and the HRP polymer for another 30 min at room temperature (Moura et al., 2012). Chromogenic development was accomplished with 3,3 0 -diaminobenzidine tetrahydrochroride (Sigma–Aldrich, Co, USA). "
ABSTRACT: This study investigated the protective effect of spray-dried açaí powder (AP) intake on colon carcinogenesis induced by 1,2-dimethylhydrazine (DMH) in male Wistar rats. After 4 weeks of DMH administrations, the groups were fed with standard diet, a diet containing 2.5% or 5.0% AP or a diet containing 0.2% N-acetylcysteine (NAC) for 10 weeks, using aberrant crypt foci (ACF) as the endpoint. Additionally, two groups were fed with standard diet or a diet containing 5.0% AP for 20 weeks, using colon tumors as the endpoint. In ACF assay, a reduction in the number of aberrant crypts (AC) and ACF (1-3 AC) were observed in the groups fed with 5.0% AP (37% AC and 47% ACF inhibition, p= 0.036) and 0.2% NAC (39% AC and 41% ACF inhibition, p= 0.042). In tumor assay, a reduction in the number of invasive tumors (p< 0.005) and tumor multiplicity (p= 0.001) was observed in the group fed with 5.0% AP. Also, a reduction in tumor Ki-67 cell proliferation (p= 0.003) and net growth index (p= 0.001) was observed in the group fed with 5.0% AP. Therefore the findings of this study indicate that AP feeding may reduce the development of chemically-induced rat colon carcinogenesis.Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 04/2013; 58. DOI:10.1016/j.fct.2013.04.011 · 2.61 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: Zinc has been proposed as a promising chemopreventive candidate against colon cancer. However, few studies on the potential beneficial effects of this trace element on cancer chemoprevention are available. The present study was designed to investigate the potential modifying influence of zinc gluconate (ZnGly) on the initiation step of colon carcinogenesis induced by 1,2-dimethylhydrazine (DMH). Male Wistar rats received orally ZnGly (15 mg elemental zinc/kg, 3 times per wk) 2 wk before and during DMH treatment (3 × 40 mg/kg, once a wk). The animals were euthanized at the end of 4th and 16th wk. Colons were analyzed for aberrant crypt foci (ACF) and tumor development. Blood and colon zinc levels, cell proliferation, and apoptosis indexes in colonic crypts were analyzed 24 h after the last DMH administration. Oral treatment with ZnGly did neither alter the number of ACF nor the indexes of cell proliferation and apoptosis in the colonic mucosa. The incidence and multiplicity of colon tumors induced by DMH and their histopathological patterns were not modified by previous treatment with ZnGly. These findings indicate a lack of chemopreventive action of zinc gluconate supplementation on the initiation step of rat colon carcinogenesis induced by DMH.Nutrition and Cancer 05/2013; 65(4):571-7. DOI:10.1080/01635581.2013.775317 · 2.47 Impact Factor