Improvement of Yeast−Biofuel Cell Output by Electrode Modifications

Industrial & Engineering Chemistry Research (Impact Factor: 2.24). 06/2010; 50(2):557–564. DOI: 10.1021/ie1000949

ABSTRACT In this study, a methodology for electrodeposition of nickel nanostructures on carbon felt was developed on the base of pulse plating technique. Different in size, shape, and distribution, Ni-island nanostructures were deposited varying the potential, current, pulse duration, and cycle reiteration. The biocompatibility and nontoxicity of the newly created materials toward Candida melibiosica yeast cells was proven. The prepared Ni-nanomodified carbon felts were investigated as anodes in a two-chamber mediatorless yeast−biofuel cell. Maximum power density values of 720 and 390 mW/m2 were achieved with the electrodes modified under galvanostatic and potentiostatic conditions, respectively, against 36 mW/m2 for the nonmodified ones. The better biofuel cell performance obtained with the Ni-modified electrodes is assigned to an improved electron transfer.

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    ABSTRACT: This paper reviews the state-of-the art of the yeast-based biofuel cell research and development. The established extracellular electron transfer (EET) mechanisms in the presence and absence of exogenous mediators are summarized and discussed. The approaches applied for improvement of mediator-less yeast-based biofuel cells performance are also presented. The overview of the literature shows that biofuel cells utilizing yeasts as biocatalysts generate power density in the range of 20 to 2440mW/m(2), which values are comparable with the power achieved when bacteria are used instead. The electrons' origin and the contribution of the glycolysis, fermentation, aerobic respiration, and phosphorylation to the EET are commented. The reported enhanced current generation in aerobic conditions presumes reconsideration of some basic MFC principles. The challenges towards the practical application of the yeast-based biofuel cells are outlined. Copyright © 2015 Elsevier B.V. All rights reserved.
    Bioelectrochemistry (Amsterdam, Netherlands) 04/2015; DOI:10.1016/j.bioelechem.2015.04.001 · 3.87 Impact Factor
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    ABSTRACT: The anaerobic fluidized bed microbial fuel cell (AFBMFC) was developed to generate electricity while simultaneously treating wastewater. During a complete cycle, the AFBMFC continuously generated electricity with a maximum power density of 1100 mW/m2 and removal of total chemical oxygen demand (COD) of 89%. To achieve this power density, the artificial electron-mediator neutral red (NR) was employed in the anode chamber. Granular biological electrodes, fluidization behavior, electron mediators, and temperature were evaluated to improve power production and wastewater treatment efficiency. The results showed that the maximum power density production of granule-graphite AFBMFC was 530 mW/m2, much higher than 410 mW/m2 using a granular activated carbon AFBMFC in the same reactor. Fluidization behaviors enhance the mass transfer and momentum transfer between activated carbon and wastewater. The power density increased with increasing methylene blue (MB) and NR concentration. Furthermore, power density reveals a slight increase as MB and NR concentrations exceed 0.5 and 1.7 mmol/L. The optimum temperature ranges from 23 to 40 °C. The Coulombic efficiency was 9.3% under the best operating conditions.
    Industrial & Engineering Chemistry Research 10/2011; 50(21):12225–12232. DOI:10.1021/ie2007505 · 2.24 Impact Factor
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    ABSTRACT: The influence of mitochondrial electron transport chain inhibitors on the electricity outputs of Candida melibiosica yeast-based biofuel cell was investigated. The addition of 30μM rotenone or antimycin A to the yeast suspension results in a decrease in the current generation, corresponding to 25.7±1.3%, respectively 38.8±1.9% reduction in the electric charge passed through the bioelectrochemical system. The latter percentage coincides with the share of aerobic respiration in the yeast catabolic processes, determined by the decrease of the ethanol production during cultivation in the presence of oxygen compared with that obtained under strict anaerobic conditions. It was established that the presence of both inhibitors leads to almost complete mitochondrial dysfunction, expressed by inactivation of cytochrome c oxidase and NADH:ubiquinone oxidoreductase as well as reduced electrochemical activity of isolated yeast mitochondria. It was also found that methylene blue partially neutralized the rotenone poisoning, probably serving as alternative intracellular electron shuttle for by-passing the complex I blockage. Based on the obtained results, we suppose that electrons generated through the aerobic respiration processes in the mitochondria participate in the extracellular electron transfer from the yeast cells to the biofuel cell anode, which contributes to higher current outputs at aerobic conditions.
    Bioelectrochemistry (Amsterdam, Netherlands) 06/2014; DOI:10.1016/j.bioelechem.2014.06.005 · 3.87 Impact Factor

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