Article

Assembly of Single-Stranded DNA Onto HOPG Surface at Different Temperature: Atomic Force Microscopy Study.

Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin, People's Republic of China; Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin, People's Republic of China.
Scanning (impact factor: 1.07). 05/2012; 34(5):302-8. DOI:10.1002/sca.21011 pp.302-8
Source: PubMed

ABSTRACT Assembly of long single-stranded DNA (ssDNA) and short oligodeoxynucleotides onto bare highly oriented pyrolytic graphite (HOPG) at different temperature has been studied. It was indicated that both long ssDNA and oligodeoxynucleotides can sequentially form network, straight chains, and layer structures when the adsorption temperature was changed from room temperature, 37-55°C. High-resolution atomic force microscopy (AFM) imaging of the layer structures revealed that they are composed of parallel ssDNA chains with relatively higher height and tend to form patterns with three-fold symmetry. These new findings are significantly important for understanding assembly characterization of ssDNA. In addition, this assembly method for ssDNA is expected to be used for preparation of DNA structures in biosensing and DNA-based nanodevices. SCANNING 34: 302-308, 2012. © 2012 Wiley Periodicals, Inc.

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Keywords

adsorption temperature
 
bare
 
biosensing
 
chains
 
different temperature
 
DNA-based nanodevices
 
High-resolution atomic force microscopy
 
higher height
 
Inc
 
layer structures
 
parallel ssDNA chains
 
room temperature
 
SCANNING 34
 
single-stranded DNA
 
ssDNA
 
three-fold symmetry
 
© 2012 Wiley Periodicals