Article

Effects of rutin on the expression of PPARγ in skeletal muscles of db/db mice.

Life Science College of the Zhejiang Chinese Medical University, Hangzhou, Zhejiang Province, China.
Planta Medica (impact factor: 2.15). 05/2012; 78(9):861-5. DOI:10.1055/s-0031-1298548 pp.861-5
Source: PubMed

ABSTRACT The effect of rutin (RT) on the expression of PPARγ in the skeletal muscle of db/db mice has been investigated. Thirty db/db mice were randomized into 5 equal groups: model control group (MCG), positive control group 30 mg/kg pioglitazone hydrochloride (PCG), low-dose rutin group 50 mg/kg (LRT), middle-dose rutin group 100 mg/kg (MRT), high-dose rutin group 200 mg/kg (HRT), and 6 other db/m mice were used as the normal control (NC). The expression of PPARγ mRNA and protein in the skeletal muscle was determined by real-time quantitative PCR and Western Blot, respectively. Treatment with HRT resulted in significant decreases in the plasma levels of glucose and lipids (p < 0.001). Compared to the MCG, the expression of PPARγ mRNA and protein of the skeletal muscle was significantly increased in the HRT and PCG groups (p < 0.001 and p < 0.05, respectively). Rutin can increase the expression of PPARγ in skeletal muscles of db/db mice.

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Keywords

5 equal groups
 
db/db mice
 
glucose
 
high-dose rutin group 200 mg/kg
 
HRT
 
low-dose rutin group 50 mg/kg
 
LRT
 
middle-dose rutin group 100 mg/kg
 
model control group
 
normal control
 
plasma levels
 
positive control group 30 mg/kg pioglitazone hydrochloride
 
PPARγ
 
PPARγ mRNA
 
real-time quantitative PCR
 
RT
 
significant decreases
 
skeletal muscle
 
skeletal muscles
 
Western Blot
 

Ying Cai