Article

Isolation of proteins from polyacrylamide gels.

Carl Gustav Carus University TU Dresden, Dresden, Germany.
Methods in molecular biology (Clifton, N.J.) 01/2012; 869:427-31. DOI:10.1007/978-1-61779-821-4_35 pp.427-31
Source: PubMed

ABSTRACT Minute amounts of proteins are required for immunization of mice for the development of antibodies including monoclonal antibodies. Here, we describe a rapid procedure for the isolation of proteins from polyacrylamide gels after sodium dodecyl sulfate polyacrylamide gel electrophoresis in sufficient amounts for immunization of animals.

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Keywords

Minute amounts
 
polyacrylamide gels
 
rapid procedure
 
sodium dodecyl sulfate polyacrylamide gel electrophoresis
 
sufficient amounts