Isolation of Proteins from Polyacrylamide Gels

Carl Gustav Carus University TU Dresden, Dresden, Germany.
Methods in molecular biology (Clifton, N.J.) (Impact Factor: 1.29). 01/2012; 869:427-31. DOI: 10.1007/978-1-61779-821-4_35
Source: PubMed


Minute amounts of proteins are required for immunization of mice for the development of antibodies including monoclonal antibodies. Here, we describe a rapid procedure for the isolation of proteins from polyacrylamide gels after sodium dodecyl sulfate polyacrylamide gel electrophoresis in sufficient amounts for immunization of animals.

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