An efficient protocol for isolating melanised chaetothyrialean anamorphic fungi associated with plant-ants

CNRS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique), Toulouse, France
Journal of Basic Microbiology (Impact Factor: 1.82). 01/2013; 52:1-3. DOI: 10.1002/jobm.201100539


Because of their ecological characteristics, slow growth rates and the presence of contaminants,
Chaetothyriales fungi associated with structures built by tropical plant-ants can be
difficult to isolate with standard procedures. Here, we describe an easy-to-use protocol for
obtaining pure cultures by using cotton as a first substrate. We have further found by means
of fluorescent stains that nuclei concentrate either in young hyphae or in the tips of the

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