Nanoemulsion Based Hydrogel Containing Omega 3 Fatty Acids as a Surrogate of Betamethasone Dipropionate for Topical Delivery
ABSTRACT The aim of the present study was to investigate the potential of nanoemulsion formulation for topical delivery of betamethasone dipropionate (BD) using fish oil (containing omega-3 fatty acids) as the oil phase. BD has anti-inflammatory, immunomodulatory and antiproliferative activity. However, its clinical use is restricted to some extent due to its poor permeability across the skin. So to increase its permeation across the skin, nanoemulsion based gel formulations were prepared and characterised. Fish oil was used as the oil phase and was also exploited for its anti-inflammatory effect along with BD in the treatment of inflammation associated with psoriasis. Nanoemulsion formulations were prepared by aqueous phase titration method, using fish oil, Unitop 100, PEG 400 and water as the oil phase, surfactant, co-surfactant and aqueous phase respectively. Furthermore, different formulations were subjected to physical stability and consequently evaluated for ex vivo permeation and in vivo anti-inflammatory study. The optimized nanoemulsion was converted into hydrogel-thickened nanoemulsion system (HTN) using carbopol 971 and had a viscosity of 98.67 ± 0.06 PaS. The optimized formulation had small average diameter (125 nm) with zeta potential of -39 mv which indicated good long-term stability. In vivo anti-inflammatory activity indicated 87.64% and 48.76% inhibition of inflammation for drug loaded and placebo formulation respectively. Assessment of skin permeation was done by ATR-FTIR, DSC and histopathology studies which indicated changes in the structure of epidermal membrane of skin.
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ABSTRACT: International Journal of Nanomedicine Dovepress submit your manuscript | www.dovepress.com Dovepress 4685 O r I g I N a l r e s e a r c h open access to scientific and medical research Open access Full Text article http://dx.doi.org/10.2147/IJN.S66180 The preparation and evaluation of self-nanoemulsifying systems containing Swietenia oil and an examination of its anti-inflammatory effects ahmad M eid 1 hesham a el-enshasy 1 ramlan aziz 1 Nagib a elmarzugi 1,2 research center, libyan authority for research, science and Technology (larsT), Tripoli, libya correspondence: Nagib a elmarzugi Abstract: There is an increasing trend among pharmaceutical industries to use natural bioactive materials as medicinal agents and to use new technologies such as self-nanoemulsifying systems. The solubility and bioavailability of poorly soluble drugs can be enhanced by self-nanoemul-sifying systems. Swietenia oil is frequently used because of its antimicrobial, antimutagenic, and anticancer bioactive medical properties. This study was conducted to develop self-nano-emulsifying systems for Swietenia oil that will enhance the anti-inflammatory activity of the oil. The self-emulsifying systems developed for Swietenia oil in this study were constructed using ternary phase diagrams and contained the nonionic surfactants Labrasol ® , Tween 20, Capmul ® , and Labrafil ® . The effect of these surfactants on the formulation was examined. The mean droplet size of Swietenia oil as well as their distribution, appearance, viscosity, and spreading times were studied to find the optimum formula, which contained droplets that were less than 200 nm. The next step was to test the anti-inflammatory properties of the optimum formula using a carrageenan-induced rat paw edema test. The results from this test were compared to the oil solution. Different oil/surfactants mixtures had various emulsification properties that were related to the size of their droplets. Tween 20 is a good surfactant to use in self-emulsifying systems because it produces droplets of nano-size. Mixtures of Capmul/Labrasol at a ratio of 2:1 and Labrafil/Tween 20 at a ratio of 1:2 were able to produce self-nanoemulsifying formu-lations containing Swietenia oil concentrations that ranged from 20%–50%. Nanoemulsion occurred when the size of the droplets fell below 200 nm with low size distribution (,0.3) after being gently mixed with water. It was found that the hydrophilic/lipophilic balance value affected the ternary phase diagram behavior of Swietenia oil and surfactants. In addition, the anti-inflammatory properties of Swietenia oil were greater in the self-nanoemulsifying systems than in the oil solution.International Journal of Nanomedicine 10/2014; 2014(1):9-4685. · 4.20 Impact Factor