Article

Mast cells in human odontogenic cysts.

Department of Oral and Maxillofacial Pathology, behind queen's college, Gram Limbodi, Indore, India.
Biotechnic & Histochemistry (Impact Factor: 0.67). 05/2012; 87(6):397-402. DOI: 10.3109/10520295.2012.674556
Source: PubMed

ABSTRACT Mast cells are granule-containing cells in mucosal and connective tissues that are known to play a central role in allergic and inflammatory responses owing to pro-inflammatory mediators. Cysts in jaws are among the most common expansive, benign and destructive bone lesions; at some stage they are associated with chronic inflammation. Earlier studies have identified mast cells in odontogenic cysts (OC). We investigated the presence and distribution of mast cells and compared their number in different types of radicular cysts (RC), dentigerous cysts (DC) and odontogenic keratocysts (OKC). Ten cases each of RC, DC and OKC diagnosed clinically and histopathologically were selected and stained with 1% toluidine blue. The greatest number of mast cells/mm(2) was found in RC. The fewest mast cells/mm(2) were found in OKC. The subepithelial zones of all cysts contained more mast cells than the deeper zones.

0 Bookmarks
 · 
133 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: The purposes of this study were a comparison of mast cells (MCs) count in oral soft-tissue reactive lesions showing different degrees of fibrosis, and also an evaluation of the correlation of mast cells counting with the score of inflammation. In this cross-sectional study, 40 samples, including irritation fibroma (IF), inflammatory fibrous hyperplasia (IFH), peripheral giant cell granuloma (PGCG), and peripheral ossifying fibroma (POF) categories (10 for each), were selected. Five normal gingival tissues were considered as the control group. The number of MCs was counted in 5 HPF containing maximum counts for each deparaffinized section stained with toluidine blue. The degree of inflammation was also evaluated for each case. The data were analyzed statistically, and P values <0.05 were considered as significant. The numbers of MCs were found to be increased in reactive lesions compared to normal gingival tissues (P value <0.05). MC counts were significantly decreased in the PGCG group compared to the IFH and POF groups (P value <0.05). A significant correlation was obtained between inflammation score and MC count in the IF and PGCG groups. (P value <0.05) The findings of this study suggest that MCs may play some role in collagen synthesis and consequently in the variation of microscopic features of oral soft-tissue reactive lesions.
    Pathology - Research and Practice 03/2010; 206(3):151-5. · 1.21 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Vascular endothelial growth factor (VEGF) expression may act as a sensitive measure of the angiogenic potential of a lesion. Furthermore, VEGF has been implicated in the pathogenesis of cystic tumors and inflammatory odontogenic cysts. Thus, we studied the expression of VEGF in the epithelium of odontogenic keratocyst (OK) in association with cell proliferation and apoptosis. Forty-two cases of OK, 26 cases of dentigerous cyst (DC), and 15 cases of residual cyst (RC) were retrospectively examined by immunohistochemistry for VEGF, Ki67/Mib-1 and anti-caspase-3. For VEGF and caspase-3, the intensity of immunostaining was qualitatively assessed, while for the evaluation of Ki67 the average number of positively stained nuclei in 10 high-power microscopic fields (x 400) was calculated. The VEGF expression was stronger in OK when compared with DC (P < 0.007). The rate of nuclear Ki67 expression in OK was significantly higher than that in DC (P < 0.001) and RC (P < 0.001). Cytoplasmic caspase-3 expression was statistically more intense in RC than in OK (P = 0.001) or DC (P < 0.001). A statistically significant correlation was seen in OK for Ki67 (P < 0.001) and VEGF (P = 0.023), but not for caspase-3. Multiple regression analysis revealed a linear relationship between VEGF and Ki67. The VEGF was expressed in the epithelium of OK, DC, and RC with a variable intensity, and in OK VEGF expression was related to Ki67. It is suggested that VEGF expression by the odontogenic epithelium is not induced solely by inflammation.
    Journal of Oral Pathology and Medicine 05/2009; 38(5):470-5. · 2.06 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to investigate the presence of mast cells in a series of odontogenic tumors. Forty-five cases of odontogenic tumors were investigated using immunohistochemistry for mast cell triptase, and differences between groups were statistically evaluated. Mast cells were present in 96% of odontogenic tumors. Mast cells present in solid ameloblastoma were observed in the tumor stroma surrounding more solid and follicular epithelial islands, with or without squamous metaplasia. The odontogenic mixoma showed few mast cells. In odontogenic tumors with a cystic structure, the mast cells were distributed throughout all areas of the lesions, mainly in keratocystic odontogenic tumor. In addition, the total density of mast cells between all odontogenic tumors showed no significant difference (p > 0.05). A greater mast cells distribution was found in keratocystic odontogenic tumor in relation to adenomatoid odontogenic tumor (p < 0.01), and when the unicystic ameloblastoma and keratocistic odontogenic tumor were compared to the odontogenic myxoma (p < 0.05). Syndrome keratocystic odontogenic tumor showed a higher mean of mast cells when compared with the other tumors of the sample. Mast cells values presented by syndrome keratocystic odontogenic tumor were significantly greater than those of the sporadic keratocystic odontogenic tumor that were not associated with the syndrome (p = 0.03). Mast cells are probably one of the major components of the stromal scaffold in odontogenic tumors. We found significant differences of mast cells between syndrome nonsyndrome keratocystic odontogenic tumors, although their distribution did not seem to have any influence on the biologic behavior of benign odontogenic tumors.
    Tumor Biology 11/2011; 33(2):455-61. · 2.52 Impact Factor