Mast cells in human odontogenic cysts.
ABSTRACT Mast cells are granule-containing cells in mucosal and connective tissues that are known to play a central role in allergic and inflammatory responses owing to pro-inflammatory mediators. Cysts in jaws are among the most common expansive, benign and destructive bone lesions; at some stage they are associated with chronic inflammation. Earlier studies have identified mast cells in odontogenic cysts (OC). We investigated the presence and distribution of mast cells and compared their number in different types of radicular cysts (RC), dentigerous cysts (DC) and odontogenic keratocysts (OKC). Ten cases each of RC, DC and OKC diagnosed clinically and histopathologically were selected and stained with 1% toluidine blue. The greatest number of mast cells/mm(2) was found in RC. The fewest mast cells/mm(2) were found in OKC. The subepithelial zones of all cysts contained more mast cells than the deeper zones.
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ABSTRACT: The aim of this study was to investigate the presence of mast cells in a series of odontogenic tumors. Forty-five cases of odontogenic tumors were investigated using immunohistochemistry for mast cell triptase, and differences between groups were statistically evaluated. Mast cells were present in 96% of odontogenic tumors. Mast cells present in solid ameloblastoma were observed in the tumor stroma surrounding more solid and follicular epithelial islands, with or without squamous metaplasia. The odontogenic mixoma showed few mast cells. In odontogenic tumors with a cystic structure, the mast cells were distributed throughout all areas of the lesions, mainly in keratocystic odontogenic tumor. In addition, the total density of mast cells between all odontogenic tumors showed no significant difference (p > 0.05). A greater mast cells distribution was found in keratocystic odontogenic tumor in relation to adenomatoid odontogenic tumor (p < 0.01), and when the unicystic ameloblastoma and keratocistic odontogenic tumor were compared to the odontogenic myxoma (p < 0.05). Syndrome keratocystic odontogenic tumor showed a higher mean of mast cells when compared with the other tumors of the sample. Mast cells values presented by syndrome keratocystic odontogenic tumor were significantly greater than those of the sporadic keratocystic odontogenic tumor that were not associated with the syndrome (p = 0.03). Mast cells are probably one of the major components of the stromal scaffold in odontogenic tumors. We found significant differences of mast cells between syndrome nonsyndrome keratocystic odontogenic tumors, although their distribution did not seem to have any influence on the biologic behavior of benign odontogenic tumors.Tumor Biology 11/2011; 33(2):455-61. · 2.52 Impact Factor
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ABSTRACT: The purposes of this study were a comparison of mast cells (MCs) count in oral soft-tissue reactive lesions showing different degrees of fibrosis, and also an evaluation of the correlation of mast cells counting with the score of inflammation. In this cross-sectional study, 40 samples, including irritation fibroma (IF), inflammatory fibrous hyperplasia (IFH), peripheral giant cell granuloma (PGCG), and peripheral ossifying fibroma (POF) categories (10 for each), were selected. Five normal gingival tissues were considered as the control group. The number of MCs was counted in 5 HPF containing maximum counts for each deparaffinized section stained with toluidine blue. The degree of inflammation was also evaluated for each case. The data were analyzed statistically, and P values <0.05 were considered as significant. The numbers of MCs were found to be increased in reactive lesions compared to normal gingival tissues (P value <0.05). MC counts were significantly decreased in the PGCG group compared to the IFH and POF groups (P value <0.05). A significant correlation was obtained between inflammation score and MC count in the IF and PGCG groups. (P value <0.05) The findings of this study suggest that MCs may play some role in collagen synthesis and consequently in the variation of microscopic features of oral soft-tissue reactive lesions.Pathology - Research and Practice 03/2010; 206(3):151-5. · 1.21 Impact Factor
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ABSTRACT: This review paper reports recent advances in the subject of developmental odontogenic cysts, essentially those of the past decade, starting with reference to the new WHO classification (1). On keratocysts, the latest reported recurrence rates are assessed as are their mode of growth, immunocytochemistry, immunology, genetic studies, and work on specific keratocyst antigens. There is a critical account of the group of lesions which includes the gingival cyst of adults, lateral periodontal cyst, hotryoid odontogenic cyst and glandular odontogenic cyst, and their possible relationship to one another. On dentigerous cysts, reference is made to the relationship between them and deciduous teeth, as well as to their immunocytochemistry and immunology. Recent work on the unicystic ameloblastomas. their classification and prognosis, is assessed, as is the calcifying odontogenic cyst and its relationship with solid odontogenic tumours.Journal of Oral Pathology and Medicine 04/2006; 23(1):1 - 11. · 2.06 Impact Factor