Rendering Protein-Based Particles Transiently Insoluble for Therapeutic Applications

Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
Journal of the American Chemical Society (Impact Factor: 11.44). 05/2012; 134(21):8774-7. DOI: 10.1021/ja302363r
Source: PubMed

ABSTRACT Herein, we report the fabrication of protein (bovine serum albumin, BSA) particles which were rendered transiently insoluble using a novel, reductively labile disulfide-based cross-linker. After being cross-linked, the protein particles retain their integrity in aqueous solution and dissolve preferentially under a reducing environment. Our data demonstrates that cleavage of the cross-linker leaves no chemical residue on the reactive amino group. Delivery of a self-replicating RNA was achieved via the transiently insoluble PRINT protein particles. These protein particles can provide new opportunities for drug and gene delivery.

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