Article

Telomerase activity alterations in sequential passages of mouse embryonic stem cells.

Laboratory of Human Reproduction, Teaching Hospital of the Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Cell Biology International (impact factor: 1.48). 05/2012; 36(8):755-7. DOI:10.1042/CBI20110591 pp.755-7
Source: PubMed

ABSTRACT Telomerase is associated with cell proliferation capacity, protection and stabilization of chromosomes. TA (telomerase activity) can be detected in highly replicative cells, e.g. stem and cancer cells. Most available mESC (mouse embryonic stem cell) research is done with a few cell lines. The purpose of this study has been to evaluate the TA in different passages of newly isolated mESC. TRAP (Telomeric Repeat Amplification Protocol)-ELISA method was used in a semi-quantitative evaluation of TA. Three mESC lineages were investigated (CT2, CT3 and CT4) at three different passages (P13, P15 and P19). In contrast with previous studies, these mESC lines did not show the same TA throughout their passages, having initially low TA values, followed by a subsequent rise and stabilization.

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Keywords

cancer cells
 
cell lines
 
cell proliferation capacity
 
chromosomes
 
different passages
 
low TA values
 
mouse embryonic
 
replicative cells
 
semi-quantitative evaluation
 
stabilization
 
subsequent rise
 
TA
 
Telomerase
 
telomerase activity
 
Telomeric Repeat Amplification Protocol)-ELISA method