Anisotropic light scattering of individual sickle red blood cells

Korea Advanced Institute of Science and Technology, Department of Physics, Daejeon 305-701, Republic of Korea.
Journal of Biomedical Optics (Impact Factor: 2.75). 04/2012; 17(4):040501. DOI: 10.1117/1.JBO.17.4.040501
Source: PubMed

ABSTRACT We present the anisotropic light scattering of individual red blood cells (RBCs) from a patient with sickle cell disease (SCD). To measure light scattering spectra along two independent axes of elongated-shaped sickle RBCs with arbitrary orientation, we introduce the anisotropic Fourier transform light scattering (aFTLS) technique and measured both the static and dynamic anisotropic light scattering. We observed strong anisotropy in light scattering patterns of elongated-shaped sickle RBCs along its major axes using static aFTLS. Dynamic aFTLS analysis reveals the significantly altered biophysical properties in individual sickle RBCs. These results provide evidence that effective viscosity and elasticity of sickle RBCs are significantly different from those of the healthy RBCs.

  • [Show abstract] [Hide abstract]
    ABSTRACT: For developing advanced query formulation methods for general multimedia data, we describe the issues related to video data. We distinguish between the requirements for image retrieval and video retrieval by identifying queryable attributes unique to video data, namely audio, temporal structure, motion, and events. Our approach is based on visual query methods to describe predicates interactively while providing feedback that is as similar as possible to the video data. An initial prototype of our visual query system for video data is presented.
    Saratov Fall Meeting 2013; 01/2014
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Rapid identification of bacterial species is crucial in medicine and food hygiene. In order to achieve rapid and label-free identification of bacterial species at the single bacterium level, we propose and experimentally demonstrate an optical method based on Fourier transform light scattering (FTLS) measurements and statistical classification. For individual rod-shaped bacteria belonging to four bacterial species (Listeria monocytogenes, Escherichia coli, Lactobacillus casei, and Bacillus subtilis), two-dimensional angle-resolved light scattering maps are precisely measured using FTLS technique. The scattering maps are then systematically analyzed, employing statistical classification in order to extract the unique fingerprint patterns for each species, so that a new unidentified bacterium can be identified by a single light scattering measurement. The single-bacterial and label-free nature of our method suggests wide applicability for rapid point-of-care bacterial diagnosis.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Due to the highly inhomogeneous distributions of refractive indexes, light propagation in complex media such as biological tissue experiences multiple light scattering events. The suppression and control of multiple light scattering events are investigated because they offer the possibility of optical focusing and imaging through biological tissues, and they may open new avenues for diagnosis and treatment of several human diseases. In order to provide insight into how new optical techniques can address the issues of multiple light scattering in biomedical applications, the recent progress in optical wavefront-shaping techniques is summarized.
    Current Applied Physics 02/2015; DOI:10.1016/j.cap.2015.02.015 · 2.03 Impact Factor

Full-text (2 Sources)

Available from
May 26, 2014