Anisotropic light scattering of individual sickle red blood cells

Korea Advanced Institute of Science and Technology, Department of Physics, Daejeon 305-701, Republic of Korea.
Journal of Biomedical Optics (Impact Factor: 2.75). 04/2012; 17(4):040501. DOI: 10.1117/1.JBO.17.4.040501
Source: PubMed

ABSTRACT We present the anisotropic light scattering of individual red blood cells (RBCs) from a patient with sickle cell disease (SCD). To measure light scattering spectra along two independent axes of elongated-shaped sickle RBCs with arbitrary orientation, we introduce the anisotropic Fourier transform light scattering (aFTLS) technique and measured both the static and dynamic anisotropic light scattering. We observed strong anisotropy in light scattering patterns of elongated-shaped sickle RBCs along its major axes using static aFTLS. Dynamic aFTLS analysis reveals the significantly altered biophysical properties in individual sickle RBCs. These results provide evidence that effective viscosity and elasticity of sickle RBCs are significantly different from those of the healthy RBCs.


Available from: YongKeun Park, Jun 07, 2015