Antibiotic resistance profile of bacterial isolates from animal farming aquatic environments and meats in a peri-urban community in South Korea

Daejeon Science High School, 19-2 Gusung-dong, Yuseong-gu, Daejeon 305-338, Korea.
Journal of Environmental Monitoring (Impact Factor: 2.18). 05/2012; 14(6):1616-21. DOI: 10.1039/c2em30168g
Source: PubMed


The increasing usage of antibiotics in the animal farming industry is an emerging worldwide problem contributing to the development of antibiotic resistance. The purpose of this work was to investigate the prevalence and antibiotic resistance profile of bacterial isolates collected from animal farming aquatic environments and meats in a peri-urban community in Daejeon, Korea. In an antibacterial susceptibility test, the bacterial isolates showed a high incidence of resistance (∼26.04%) to cefazolin, tetracycline, gentamycin, norfloxacin, erythromycin and vancomycin. The results from a test for multiple antibiotic resistance indicated that the isolates were displaying an approximately 5-fold increase in the incidence of multiple antibiotic resistance to combinations of two different antibiotics compared to combinations of three or more antibiotics. Most of the isolates showed multi-antibiotic resistance, and the resistance patterns were similar among the sampling groups. Sequencing data analysis of 16S rRNA showed that most of the resistant isolates appeared to be dominated by the classes Betaproteobacteria and Gammaproteobacteria, including the genera Delftia, Burkholderia, Escherichia, Enterobacter, Acinetobacter, Shigella and Pseudomonas.

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    • "The colony forming units (CFU) of cultured bacteria were counted and the antibiotic resistance colony number was determined. The percentage resistance of each treatment was counted as the ratio between the number of colonies on beef extract peptone agar medium with and without penicillin, multiplied by 100 (Rho et al., 2012). "

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    • "And multidrug resistant (MDR) transporters that expel drugs from the cytoplasm or cytoplasmic membrane to the external medium constitute one of the proven causes of arrival of MDR bacteria, as demonstrated in E. coli [4] . Camaraderie in bacteria (both pathogenic and non-pathogenic) help exchange of genetic materials in aggrandizement of multidrug resistance, from one another [3] , eventually landing at intractable clinical managements with bacterial pathogens [5] . Cataclysmic rather staggering emergence of pandrug resistance ( resistance to all of antibiotics in use) is recorded in E. coli, Pseudomonas aeruginosa, Acinetobacter baumannii and Klebsiella pneumoniae [6] , that mire patients to a spate of comorbidities, as if living in a hospice, challenging the clinical or rather cleanly totem pole of a hospital. "
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