A PR-1-like Protein of Fusarium oxysporum Functions in Virulence on Mammalian Hosts

Departamento de Genetica, Facultad de Ciencias and Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Cordoba, 14071 Cordoba, Spain.
Journal of Biological Chemistry (Impact Factor: 4.57). 05/2012; 287(26):21970-9. DOI: 10.1074/jbc.M112.364034
Source: PubMed


The pathogenesis-related PR-1-like protein family comprises secreted proteins from the animal, plant, and fungal kingdoms
whose biological function remains poorly understood. Here we have characterized a PR-1-like protein, Fpr1, from Fusarium oxysporum, an ubiquitous fungal pathogen that causes vascular wilt disease on a wide range of plant species and can produce life-threatening
infections in immunocompromised humans. Fpr1 is secreted and proteolytically processed by the fungus. The fpr1 gene is required for virulence in a disseminated immunodepressed mouse model, and its function depends on the integrity of
the proposed active site of PR-1-like proteins. Fpr1 belongs to a gene family that has expanded in plant pathogenic Sordariomycetes.
These results suggest that secreted PR-1-like proteins play important roles in fungal pathogenicity.

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    • "F. oxysporum has been studied in detail as a plant pathogen and is attracting increasing interest as a model for cross-kingdom pathogenicity in fungi [5]. Analysis of knockout mutants in the mouse model revealed striking similarities of infection mechanisms with other well-established human pathogens [3], [5], [6], [7], [8]. "
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    ABSTRACT: Fusarium oxysporum is an important plant pathogen and an opportunistic pathogen of humans. Here we investigated phagocytosis of F. oxysporum by J774.1 murine cell line macrophages using live cell video microscopy. Macrophages avidly migrated towards F. oxysporum germlings and were rapidly engulfed after cell-cell contact was established. F. oxysporum germlings continued hyphal growth after engulfment by macrophages, leading to associated macrophage lysis and escape. Macrophage killing depended on the multiplicity of infection. After engulfment, F. oxysporum inhibited macrophages from completing mitosis, resulting in large daughter cells fused together by means of a F. oxysporum hypha. These results shed new light on the initial stages of Fusarium infection and the innate immune response of the mammalian host.
    PLoS ONE 07/2014; 9(7):e101999. DOI:10.1371/journal.pone.0101999 · 3.23 Impact Factor
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    • "The ability to cause disease in both plants and mammals makes F. oxysporum a unique multihost pathogen for studying fungal infection across different host kingdoms. To date several knockout mutants have been tested for virulence in immunosuppressed mice [19], [20], [21], [22], [23]. "
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    ABSTRACT: The soil-borne plant pathogen Fusarium oxysporum causes life-threatening invasive fusariosis in immunocompromised individuals. The mechanism of infection in mammalian hosts is largely unknown. In the present study we show that the symptoms of disseminated fusariosis caused by F. oxysporum in immunosuppressed mice are remarkably similar to those reported in humans. Distinct fungal structures were observed inside the host, depending on the infected organ. Invasive hyphae developed in the heart and kidney, causing massive colonization of the organs. By contrast, chlamydospore-like survival structures were found in lung, spleen and liver. Systemically infected mice also developed skin and eye infections, as well as thrombosis and necrosis in the tail. We further show that F. oxysporum can disseminate and persist in the organs of immunocompetent animals, and that these latent infections can lead to lethal systemic fusariosis if the host is later subjected to immunosuppressive treatment.
    PLoS ONE 02/2014; 9(2):e89920. DOI:10.1371/journal.pone.0089920 · 3.23 Impact Factor
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    • "Interestingly, the same region was found to have a loss of heterozygosity relative to UM142 and the GP clade. It has been suggested that these genes are involved in the pathogenesis and virulence of plant and animals pathogens, and may play a role in spore penetration and modulation of the host defense response [49,50]. While these results should be treated with healthy skepticism, due to a variety of possible difficulties, such as the observed high rate of copy-number variations in the Bd chromosomes, they illustrate the kinds of tantalizing observations that can be made with our Galaxy tools. "
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    ABSTRACT: Intra-species genetic variation can be used to investigate population structure, selection, and gene flow in non-model vertebrates; and due to the plummeting costs for genome sequencing, it is now possible for small labs to obtain full-genome variation data from their species of interest. However, those labs may not have easy access to, and familiarity with, computational tools to analyze those data. We have created a suite of tools for the Galaxy web server aimed at handling nucleotide and amino-acid polymorphisms discovered by full-genome sequencing of several individuals of the same species, or using a SNP genotyping microarray. In addition to providing user-friendly tools, a main goal is to make published analyses reproducible. While most of the examples discussed in this paper deal with nuclear-genome diversity in non-human vertebrates, we also illustrate the application of the tools to fungal genomes, human biomedical data, and mitochondrial sequences. This project illustrates that a small group can design, implement, test, document, and distribute a Galaxy tool collection to meet the needs of a particular community of biologists.
    12/2013; 2(1):17. DOI:10.1186/2047-217X-2-17
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