Genetic variability evaluation in a Moroccan collection of barley, Hordeum vulgare L., by means of storage proteins and RAPDs

Genetic Resources and Crop Evolution (Impact Factor: 1.48). 03/2002; 49:619-631. DOI: 10.1023/A:1021228730714

ABSTRACT The genetic variation existing in a set of barley (Hordeum vulgare L.) landrace samples recently collected in
Morocco was estimated. Two kinds of genetic markers, seed storage proteins (hordeins) and random amplified
polymorphic DNA (RAPD), were used. Only six out of 31 landraces were subjected to RAPD analysis. Both kinds
of markers, RAPD and storage proteins, yielded similar results, showing that the level of variation observed in
Moroccan barley was high: all landraces showed variability; 808 different storage protein patterns (multilocus
associations) were observed among 1897 individuals (2.32 seeds per association, on average) with an average of
43 multilocus associations per accession. In general, genetic variation within accessions was higher than between
accessions. The 100 polymorphic RAPD bands generated by 21 effective primers were able to generate enough
patterns to differentiate between uniform cultivars and even between individuals in variable accessions. One of the
aims of this work was to compare the effectiveness of RAPD versus storage protein techniques in assessing the
variability of genetic resource collections. On average hordeins were more polymorphic than RAPDs: they showed
more alternatives per band on gels and a higher percentage of polymorphic bands, although RAPDs supply a
higher number of bands. Although RAPD is an easy and standard technique, storage protein analysis is technically
easier, cheaper and needs less sophisticated equipment. Thus, when resources are a limiting factor and considering
the cost of consumables and work time, seed storage proteins must be the technique of choice for a first estimation
of genetic variation in plant genetic resource collections.

  • [Show abstract] [Hide abstract]
    ABSTRACT: In this study, starch gel electrophoresis was used to examine polymorphism of hordeins encoded by the Hrd A, Hrd B, and Hrd F loci in 201 accessions of barley landraces from China (including Tibet), Nepal, Pakistan, and India. Altogether, 50 alleles with the frequencies of 0.001–0.2269 were determined for the Hrd A locus, 65 alleles with the frequencies of 0.001–0.1612 were determined for the Hrd B locus, and five alleles with the frequencies of 0.001–0.4537 were determined for the Hrd F locus. In barley populations from these countries, irregular distribution of alleles and allele frequencies was observed. Cluster analysis of the matrix of allele frequencies in populations from known sampling sites revealed cluster structure of local barley populations within each country. Local populations formed five differently sized clusters in Nepal, four such clusters in India, three clusters in China, and three clusters, in Pakistan. These results suggest that variation and allele frequency distribution of the hordein-coding loci in the countries of Eastern Asia resulted from the introduction and spreading of barley forms through the husbandmen migrations.
    Russian Journal of Genetics 08/2012; 48(8). · 0.41 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This study was conducted to determine the effects of salinity on seed germination and early vegetative growth of nine genotypes of barley (5 landraces & 4 breeding lines). The genotypes were evaluated by several criteria, at four salt concentrations (0, 100, 150 & 200 mM) and four seawater concentrations (0, 20, 30 & 40%). The results revealed a large variability within the genotypes for salt tolerance at the two early growth stages. Genotype x treatment interaction was significant for root length and highly significant for the dry weight of roots. The levels of salt tolerance in some barley landraces were higher than those found in breeding lines, particularely for seed germination. Our results indicate that there was no relationship between salt tolerance during seed germination and during early vegetative growth.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) were assayed to determine the genetic diversity of 80 barley specimens from South Tunisia. The ISSR primers showed variation in the percentage of polymorphism, band informativeness (Ib), and resolving power (Rp). The percentage of polymorphism is 66.67%, the average Ib ranged from 0.24 to 0.39, while Rp ranged from 0.74 to 1.16. In RAPD analysis, three primers yielded a total of 17 scorable bands, which are all polymorphic. The three polymorphic primers exhibited variation with regard to average band informativeness (AvIb) and resolving power (Rp). RAPD and ISSR marker systems were found to be useful for the genetic diversity among the barley specimens. The two dendrograms obtained through these markers show different clustering of 80 barely specimens, but we noted that some clusters were similar in some cases. A poor correlation (𝑟=0.12) was found between both sets of genetic similarity data, suggesting that both sets of markers revealed unrelated estimates of genetic relationships. Therefore, the ISSR and RAPD molecular markers show two genetic grouping of studied barely specimens.
    ISRN Agronomy. 01/2012; 2012.