Genetic variability evaluation in a Moroccan collection of barley, Hordeum vulgare L., by means of storage proteins and RAPDs

Genetic Resources and Crop Evolution (Impact Factor: 1.59). 03/2002; 49:619-631. DOI: 10.1023/A:1021228730714

ABSTRACT The genetic variation existing in a set of barley (Hordeum vulgare L.) landrace samples recently collected in
Morocco was estimated. Two kinds of genetic markers, seed storage proteins (hordeins) and random amplified
polymorphic DNA (RAPD), were used. Only six out of 31 landraces were subjected to RAPD analysis. Both kinds
of markers, RAPD and storage proteins, yielded similar results, showing that the level of variation observed in
Moroccan barley was high: all landraces showed variability; 808 different storage protein patterns (multilocus
associations) were observed among 1897 individuals (2.32 seeds per association, on average) with an average of
43 multilocus associations per accession. In general, genetic variation within accessions was higher than between
accessions. The 100 polymorphic RAPD bands generated by 21 effective primers were able to generate enough
patterns to differentiate between uniform cultivars and even between individuals in variable accessions. One of the
aims of this work was to compare the effectiveness of RAPD versus storage protein techniques in assessing the
variability of genetic resource collections. On average hordeins were more polymorphic than RAPDs: they showed
more alternatives per band on gels and a higher percentage of polymorphic bands, although RAPDs supply a
higher number of bands. Although RAPD is an easy and standard technique, storage protein analysis is technically
easier, cheaper and needs less sophisticated equipment. Thus, when resources are a limiting factor and considering
the cost of consumables and work time, seed storage proteins must be the technique of choice for a first estimation
of genetic variation in plant genetic resource collections.

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    ABSTRACT: Genetic diversity among 63 hulless barley accessions originating from ICARDA (International Centre for Agricultural Research in the Dry Area) was investigated using agromorphological traits and random amplified polymorphic DNA (RAPD) variation. Among 20 agromorphological traits under study, a considerable diversity was observed for grain yield per plot, 1000-grain weight and peduncle length. High estimates of heritability in broad sense were recorded for plant height, number of grains per spike and number of tillers. These traits can be used for indirect improvement of yield. Cluster and Principal component analysis based on agromorphological traits could effectively classify the samples. The RAPD-based genetic similarity ranged from 0.221 to 0.81, with the mean of 0.481. Cluster analysis based on Jaccard Similarity Coefficient divided genotypes into 8 different groups. The average of genetic diversity index for RAPDs and storage proteins were compared and showed that mean of genetic diversity index was lesser for RAPDs than storage proteins. Thus, when resources are a limiting factor and considering the cost of consumables and work time, seed storage proteins must be the technique of choice for a first estimation of genetic variation in hulless barley genetic resource collections. In RAPD analysis, a special band was observed which could be used in identifying hulless barley varieties with low or high β β β β-glucan.
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    ABSTRACT: Random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) were assayed to determine the genetic diversity of 80 barley specimens from South Tunisia. The ISSR primers showed variation in the percentage of polymorphism, band informativeness (Ib), and resolving power (Rp). The percentage of polymorphism is 66.67%, the average Ib ranged from 0.24 to 0.39, while Rp ranged from 0.74 to 1.16. In RAPD analysis, three primers yielded a total of 17 scorable bands, which are all polymorphic. The three polymorphic primers exhibited variation with regard to average band informativeness (AvIb) and resolving power (Rp). RAPD and ISSR marker systems were found to be useful for the genetic diversity among the barley specimens. The two dendrograms obtained through these markers show different clustering of 80 barely specimens, but we noted that some clusters were similar in some cases. A poor correlation (𝑟=0.12) was found between both sets of genetic similarity data, suggesting that both sets of markers revealed unrelated estimates of genetic relationships. Therefore, the ISSR and RAPD molecular markers show two genetic grouping of studied barely specimens.
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