Genetic variability evaluation in a Moroccan collection of barley, Hordeum vulgare L., by means of storage proteins and RAPDS

Genetic Resources and Crop Evolution (Impact Factor: 1.46). 03/2002; 49:619-631. DOI: 10.1023/A:1021228730714


The genetic variation existing in a set of barley (Hordeum vulgare L.) landrace samples recently collected in
Morocco was estimated. Two kinds of genetic markers, seed storage proteins (hordeins) and random amplified
polymorphic DNA (RAPD), were used. Only six out of 31 landraces were subjected to RAPD analysis. Both kinds
of markers, RAPD and storage proteins, yielded similar results, showing that the level of variation observed in
Moroccan barley was high: all landraces showed variability; 808 different storage protein patterns (multilocus
associations) were observed among 1897 individuals (2.32 seeds per association, on average) with an average of
43 multilocus associations per accession. In general, genetic variation within accessions was higher than between
accessions. The 100 polymorphic RAPD bands generated by 21 effective primers were able to generate enough
patterns to differentiate between uniform cultivars and even between individuals in variable accessions. One of the
aims of this work was to compare the effectiveness of RAPD versus storage protein techniques in assessing the
variability of genetic resource collections. On average hordeins were more polymorphic than RAPDs: they showed
more alternatives per band on gels and a higher percentage of polymorphic bands, although RAPDs supply a
higher number of bands. Although RAPD is an easy and standard technique, storage protein analysis is technically
easier, cheaper and needs less sophisticated equipment. Thus, when resources are a limiting factor and considering
the cost of consumables and work time, seed storage proteins must be the technique of choice for a first estimation
of genetic variation in plant genetic resource collections.

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Available from: EL Habib Dakir, Jun 24, 2015
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    • "Of these techniques, RAPD have become one of the most widely used marker systems in studies related to plant genetic resources. They yield a high number of discrete bands and have been extensively used to document genetic variation in hulless and hulled barley (Chalmers et al., 1993; Tinker et al., 1993; Papa et al., 1998; Hong et al., 2001; Santos et al., 2001; Dakir et al., 2002; Yu et al., 2002; Yong-Cui et al., 2005; Michelmore et al., 2007). "
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    ABSTRACT: Genetic diversity among 63 hulless barley accessions originating from ICARDA (International Centre for Agricultural Research in the Dry Area) was investigated using agromorphological traits and random amplified polymorphic DNA (RAPD) variation. Among 20 agromorphological traits under study, a considerable diversity was observed for grain yield per plot, 1000-grain weight and peduncle length. High estimates of heritability in broad sense were recorded for plant height, number of grains per spike and number of tillers. These traits can be used for indirect improvement of yield. Cluster and Principal component analysis based on agromorphological traits could effectively classify the samples. The RAPD-based genetic similarity ranged from 0.221 to 0.81, with the mean of 0.481. Cluster analysis based on Jaccard Similarity Coefficient divided genotypes into 8 different groups. The average of genetic diversity index for RAPDs and storage proteins were compared and showed that mean of genetic diversity index was lesser for RAPDs than storage proteins. Thus, when resources are a limiting factor and considering the cost of consumables and work time, seed storage proteins must be the technique of choice for a first estimation of genetic variation in hulless barley genetic resource collections. In RAPD analysis, a special band was observed which could be used in identifying hulless barley varieties with low or high β β β β-glucan.
    African journal of agricultural research 02/2010; 5(1):97-107. · 0.26 Impact Factor
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    • "Firstly, there must be significant variation for the character to be improved and secondly, the character must be affected by genetic component. In the previous reports, presence of variation in salt tolerance had been observed in different crops, as for example, in wheat (Akhtar et al., 2003; Bhatti et al., 2004; Ali et al., 2007), maize (Rao & McNeilly, 1999; Khan et al., 2003), sorghum (Azhar & McNeilly, 1987, 2000 & 2001), sunflower (Bhutta et al., 2004), barley (Czembor, 2000; Dakir et al., 2002; Madidi et al., 2004), rice (Ahmad et al.,1990; Shannon et al., 1998; Lee et al., 2003). Cotton (Gossypium hirsutum L.) is an important cash crop grown in the area mostly affected by salinity in the country. "
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    ABSTRACT: The genetic basis of salt (NaCl) tolerance at maturity stage was examined in six accessions of Gossypium hirsutum L. at plant maturity. The six accessions were crossed in all possible combinations. The NaCl tolerance of 30 F 1 hybrids and their six parents were assessed at maturity level in the iron containers by subjecting to constant NaCl treatments i.e., 0, 17.5, and 20 dS/m salinity. Indices of salt tolerance (relative salt tolerance) were analyzed using diallel method. Both additive and dominance effects appeared to be important for the expression of variation under low and high salinity levels. Estimates of narrow sense heritabilities for salt tolerance were remarkable. This suggested that rapid improvement in (NaCl) tolerance in G. hirsutum using high selection pressures in the F 2 population may be made through selection and breeding.
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    ABSTRACT: This study was conducted to determine the effects of salinity on seed germination and early vegetative growth of nine genotypes of barley (5 landraces & 4 breeding lines). The genotypes were evaluated by several criteria, at four salt concentrations (0, 100, 150 & 200 mM) and four seawater concentrations (0, 20, 30 & 40%). The results revealed a large variability within the genotypes for salt tolerance at the two early growth stages. Genotype x treatment interaction was significant for root length and highly significant for the dry weight of roots. The levels of salt tolerance in some barley landraces were higher than those found in breeding lines, particularely for seed germination. Our results indicate that there was no relationship between salt tolerance during seed germination and during early vegetative growth.
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