Article

Determination of creatine and phosphocreatine in muscle biopsy samples by capillary electrophoresis with contactless conductivity detection.

Department of Chemistry, University of Basel, Spitalstrasse 51, 4056 Basel, Switzerland.
Analytica chimica acta (impact factor: 4.31). 05/2012; 727:78-82. DOI:10.1016/j.aca.2012.03.055
Source: PubMed

ABSTRACT A capillary electrophoresis method with contactless conductivity detection was evaluated as a new approach for quantification of creatine and phosphocreatine in human quadriceps femoris biopsy samples. The running buffers employed consisted of 1 M acetic acid at a pH of 2.3 for the determination of creatine and 50 mM 3-(N-morpholino)propanesulfonic acid/30 mM histidine at a pH of 6.4 for the determination of phosphocreatine in the centrifuged muscle extracts. The limits of detection for creatine and phosphocreatine were found to be 2.5 and 1.0 μM, respectively. Creatine and phosphocreatine were determined in six human muscle biopsy samples and the results were found comparable to those of a standard enzymatic assay. The procedures developed for creatine and phosphocreatine also allow the determination of creatinine as well as adenosine diphosphate and adenosine triphosphate.

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Keywords

1 M acetic acid
 
adenosine triphosphate
 
capillary electrophoresis method
 
centrifuged muscle
 
comparable
 
contactless conductivity detection
 
creatinine
 
detection
 
human muscle biopsy samples
 
human quadriceps femoris biopsy samples
 
limits
 
phosphocreatine
 
procedures
 
quantification
 
running buffers
 
standard enzymatic assay
 
μM
 

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