In vivo and in vitro effect of bendiocarb on rabbit testicular structure and spermatozoa motility.
ABSTRACT In this study the effect of bendiocarb on the rabbit testicular structure and spermatozoa motility was investigated. For testicular structure evaluation the animals were fed with bendiocarb tablets daily at a dose of 5 mg/kg of body weight for 13 days. The relative volume of the germinal epithelium, interstitium and lumen was measured. The testicular structure evaluation showed decreased relative volume of germinal epithelium in both experimental groups in comparison with the control group. The relative volume of the interstitium was increased in both experimental groups. An increase of the relative volume of the lumen was registered also in both experimental groups. Qualitative analysis detected a dilatation of blood vessels in the interstitium, undulation of the basal membrane and some empty spaces in the germinal epithelium after bendiocarb administration. The spermatozoa motility was evaluated by the computer assisted semen analyzer (CASA) method in various time intervals (0-180 minutes) and the bendiocarb concentration in the culture medium added to experimental groups varied from 0.054 to 0.268 mg/mL. Spermatozoa motility and progressive motility significantly decreased with increased bendiocarb administration and with extending the period of incubation. For other fine motility parameters, a decrease dependent on the time of incubation and on the bendiocarb concentration almost in all experimental groups in comparison to the control was detected. These results clearly suggest that in vitro also in vivo bendiocarb administration decrease male fertility.
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ABSTRACT: Citation: Falana BA, Ogundele OM, Duru FI, Osinubi AA, Falode DT (2013) Immunohistochemical Characterization of Lymphocytic CD3/CD20 and Macrophage CD68 in the Germinal Epithelium of Pb and Se+Zn Treated Adult Sprague-Dawley Rats. J Cytol Histol 4: 171. Abstract Lead toxicity in the testes has been described to be capable of inducing cell death by apoptosis and necrosis. Such toxicity can be attenuated by selenium and zinc synergy treatment in trace amount. This study evaluates the role and distribution of macrophages/histocytes (CD68), B-Lymphocytes (CD20) and T-Lymphocytes (CD3) in the testes of lead, selenium and zinc treated rats. 60 F1 generation adult male Sprague-Dawley rats were divided into four groups of 15 animals each. Group 1 received normal saline, group 2: 100 mg/Kg of lead acetate, group 3: 100 mg/kg of lead acetate then 2.25 mg/ Kg each of Zinc (Chelated zinc) and Selenium (Sodium Selenium) and group 4: 2.25 mg/kg of zinc and selenium (Se+Zn). The duration of treatment was 56 days following which the animals were sacrificed on the 57 th day and the testes harvested and fixed in Bouin's fluid. CD3, CD20 and CD68 are distributed within the epithelium and the interstitium of the Pb treated testis, the expression level is influenced by the extent of the damage posed by Pb toxicity and not by the proliferative tendencies of Se+Zn treatment did protect the germinal epithelium and the macrophage/lymphocyte cell lines.Journal of Cytology 04/2013; 4(2). · 0.31 Impact Factor