In vivo and in vitro effect of bendiocarb on rabbit testicular structure and spermatozoa motility
ABSTRACT In this study the effect of bendiocarb on the rabbit testicular structure and spermatozoa motility was investigated. For testicular structure evaluation the animals were fed with bendiocarb tablets daily at a dose of 5 mg/kg of body weight for 13 days. The relative volume of the germinal epithelium, interstitium and lumen was measured. The testicular structure evaluation showed decreased relative volume of germinal epithelium in both experimental groups in comparison with the control group. The relative volume of the interstitium was increased in both experimental groups. An increase of the relative volume of the lumen was registered also in both experimental groups. Qualitative analysis detected a dilatation of blood vessels in the interstitium, undulation of the basal membrane and some empty spaces in the germinal epithelium after bendiocarb administration. The spermatozoa motility was evaluated by the computer assisted semen analyzer (CASA) method in various time intervals (0-180 minutes) and the bendiocarb concentration in the culture medium added to experimental groups varied from 0.054 to 0.268 mg/mL. Spermatozoa motility and progressive motility significantly decreased with increased bendiocarb administration and with extending the period of incubation. For other fine motility parameters, a decrease dependent on the time of incubation and on the bendiocarb concentration almost in all experimental groups in comparison to the control was detected. These results clearly suggest that in vitro also in vivo bendiocarb administration decrease male fertility.
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ABSTRACT: Citation: Falana BA, Ogundele OM, Duru FI, Osinubi AA, Falode DT (2013) Immunohistochemical Characterization of Lymphocytic CD3/CD20 and Macrophage CD68 in the Germinal Epithelium of Pb and Se+Zn Treated Adult Sprague-Dawley Rats. J Cytol Histol 4: 171. Abstract Lead toxicity in the testes has been described to be capable of inducing cell death by apoptosis and necrosis. Such toxicity can be attenuated by selenium and zinc synergy treatment in trace amount. This study evaluates the role and distribution of macrophages/histocytes (CD68), B-Lymphocytes (CD20) and T-Lymphocytes (CD3) in the testes of lead, selenium and zinc treated rats. 60 F1 generation adult male Sprague-Dawley rats were divided into four groups of 15 animals each. Group 1 received normal saline, group 2: 100 mg/Kg of lead acetate, group 3: 100 mg/kg of lead acetate then 2.25 mg/ Kg each of Zinc (Chelated zinc) and Selenium (Sodium Selenium) and group 4: 2.25 mg/kg of zinc and selenium (Se+Zn). The duration of treatment was 56 days following which the animals were sacrificed on the 57 th day and the testes harvested and fixed in Bouin's fluid. CD3, CD20 and CD68 are distributed within the epithelium and the interstitium of the Pb treated testis, the expression level is influenced by the extent of the damage posed by Pb toxicity and not by the proliferative tendencies of Se+Zn treatment did protect the germinal epithelium and the macrophage/lymphocyte cell lines.Journal of Cytology 04/2013; 4(2). DOI:10.4172/2157-7099.1000171 · 0.37 Impact Factor
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ABSTRACT: The aim of this study was to evaluate the turkey spermatozoa motility in in vitro conditions and to prove the effect of different conditions of incubation – diluents, temperature and age of birds. Spermatozoa were obtained from adult turkey's line of Big 6, and spermatozoa motility parameters were evaluated using a computer-assisted semen analyzer (CASA) system. Significant decrease of spermatozoa motility at laboratory temperature (22°C) was detected from time 0 (94.15%) till 180 minutes of incubation (53.91%). At the cool media incubation (5°C), this difference was lower (95.41 and 78.86%, respectively), and the differences were significant from 30 minutes of incubation till 180 minutes. Progressive spermatozoa motility replicated the tendency of total spermatozoa motility. When the physiological solution to commercial diluent at 5°C was compared, the spermatozoa motility and progressive motility in both groups were very consistent for 90 minutes of incubation. Subsequently, significantly higher spermatozoa motility was detected at time periods 120, 150 and 180 minutes of incubation in commercial diluent. Motility was also higher in this group after 24 hours. Influence of age on spermatozoa motility parameters was analysed at 22°C at the time 0 and after 30 minutes of incubation. Analysis of spermatozoa motility as well as progressive spermatozoa motility proved higher values in Group A (aged 35–42 weeks) compared to Group B (aged 63–73 weeks). These results clearly suggest that low temperature and commercial diluents maintain motility parameters during longer time periods and the increasing age of birds has negative impact on motility parameters.Journal of Applied Animal Research 12/2014; 43(2):1-6. DOI:10.1080/09712119.2014.928627 · 0.44 Impact Factor
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ABSTRACT: This in vitro study was designed to assess the impact of divalent (Fe(2+)) or trivalent (Fe(3+)) iron on the activity and oxidative balance of bovine spermatozoa at specific time intervals (0, 2, 8, 16, and 24 h) during an in vitro culture. Forty-five semen samples were collected from adult breeding bulls and diluted in physiological saline solution supplemented with different concentrations (0, 1, 5, 10, 50, 100, 200, 500, 1000 μmol/L) of FeCl2 or FeCl3. Spermatozoa motion parameters were assessed using the SpermVision™ computer-aided sperm analysis (CASA) system. Cell viability was examined with the metabolic activity 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the nitroblue-tetrazolium (NBT) test was applied to quantify the intracellular superoxide formation. Both divalent and trivalent iron exhibited a dose- and time-dependent impact on the spermatozoa physiology and oxidative balance. Concentrations ≥50 μmol/L FeCl2 and ≥100 μmol/L FeCl3 led to a significant decrease of spermatozoa motility (P < 0.05) and mitochondrial activity (P < 0.001 with respect to 200-1000 μmol/L FeCl2/FeCl3; P < 0.01 in case of 100 μmol/L FeCl2/FeCl3), accompanied by a significant superoxide overproduction (P < 0.001 in terms of 200-1000 μmol/L FeCl2 and 500-1000 μmol/L FeCl3; P < 0.01 with respect to 100 μmol/L FeCl2 and 100-200 μmol/L FeCl3). On the other hand, concentrations below 10 μmol/L FeCl2 and 50 μmol/L FeCl3 proved to stimulate the spermatozoa activity, as shown by a significant preservation of the motility and viability characteristics (P < 0.001 in case of the motility parameters; P < 0.01 with respect to the spermatozoa viability), alongside a significant decline of the superoxide generation (P < 0.05). In a direct comparison, divalent iron has been shown to be more toxic than trivalent iron. Results from this in vitro study show that high concentrations of both forms of iron are toxic, while their low concentrations may have spermatozoa activity-promoting properties. In vitro concentrations of divalent or trivalent iron that could be regarded as critical are 50 μmol/L FeCl2 and 100 μmol/L FeCl3 when iron ceases to be an essential micronutrient in order to become a toxic risk factor.Biological Trace Element Research 03/2015; 167(1). DOI:10.1007/s12011-015-0288-5 · 1.75 Impact Factor