Royal jelly modulates oxidative stress and tissue injury in gamma irradiated male Wister Albino rats
ABSTRACT Royal jelly is a nutritive secretion produced by the worker bees, rich in proteins, carbohydrates, vitamins and minerals.
The present study was designed to determine the possible protective effects of royal jelly against radiation induced oxidative stress, hematological, biochemical and histological alterations in male Wister albino rats.
Male Wister albino rats were exposed to a fractionated dose of gamma radiation (2 Gy every 3 days up to 8 Gy total doses). Royal jelly was administrated (g/Kg/day) by gavages 14 days before exposure to the 1(st) radiation fraction and the treatment was continued for 15 days after the 1(st) irradiation fraction till the end of the experiment. The rats were sacrificed 3(rd), equivalent to 3rd post 2nd irradiation fraction, and equivalent to 3rd day post last irradiation fraction.
In the present study, gamma- irradiation induced hematological, biochemical and histological effects in male Wister albino rats. In royal jelly treated irradiated group, there was a noticeable decrease recorded in thiobarbituric reactive substances concentration when compared to γ-irradiated group. Also, the serum nitric oxide concentration was significantly improved. The administration of royal jelly to irradiated rats according to the current experimental design significantly ameliorates the changes induced in serum lipid profile. Moreover, in royal jelly treated irradiated group, there was a noticeable amelioration recorded in all hematological parameters along the three experimental intervals. The microscopic examination of cardiac muscle of royal jelly treated irradiated rats demonstrated structural amelioration, improved nuclei and normal features of capillaries and veins in endomysium when compared to gamma-irradiated rats.
It was suggested that the biochemical, hematological and histological amelioration observed in royal jelly (g/Kg/day) treated irradiated rats might be due to the antioxidant capacity of royal jelly active constituents.
Full-textDOI: · Available from: Hamed Abdelazim Helal, May 30, 2015
SourceAvailable from: Khaled Azab
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ABSTRACT: Systemic inflammation could be one of the serious cascades of oxidative stress developed after exposure to ionizing radiation. The present work was to elucidate the possible role of cranberry extract against inflammatory responses induced in gamma irradiated rats. The experimental animals were exposed to whole body gamma radiation (2 Gy every 3 days up to total dose of 8 Gy) at dose rate 0.48 Gy/min and received daily oral administration of 0.5ml cranberry extract (≈100 mg/kg/day) for 14 consecutive days before starting irradiation and the treatment was extended during exposure to radiation fractions. Rats were classified into four groups:1) normal control, 2)irradiated, 3)cranberry, and 4) irradiated + cranberry. The data obtained reveals significant improvement in inflammatory parameters (TNF-α, ADAM12, MMP2 and MMP9 in rats treated with cranberry and exposed to gamma irradiation when compared with irradiated rats. In addition, significant ameliorations in liver antioxidant parameters (SOD, CAT and GSH) were noticed in cranberry + irradiated rats. These results were accompanied with remarkable decreases in Liver TBARS and NO contents. It could be suggested that cranberry extract neutralize the systemic inflammatory response resulted from radiation exposure throughout the amendment of antioxidant status and oxidative stress diminution.
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ABSTRACT: Ethnopharmacological relevance: Royal Jelly (RJ) is a bee-derived product that has been traditionally used in the European and Asian systems of medicine for longevity. RJ has various pharmacological activities that may prevent aging e.g., anti-inflammatory, anti-oxidative, anti-hypercholesterolemic and anti-hyperglycemic properties. Aim of the study: To evaluate the behavioral and neurochemical effects of long-term oral, previously chemically analyzed, Greek RJ administration to aged rats. Materials and methods: RJ powder was given to 18-month old male Wistar rats (50 and 100 mg of powder/kg b.w./day) by gastric gavage for 2 months. The spatial memory was assessed in the water maze and next the level of neurotransmitters, their metabolites and utilization in the selected brain regions were estimated. Results: The improvement of memory in rats pretreated with the smaller dose of RJ was observed compared with controls. In biochemical examination mainly the depletion of dopamine and serotonin in the prefrontal cortex along with an increase in their metabolite concentration and turnover were seen. Conclusion: Better cognitive performance in the old animals using a non-toxic, natural food product in the view of the process of the aging of human population is noteworthy. Our results contribute towards validation of the traditional use of RJ in promoting a better quality of life in old age.Journal of Ethnopharmacology 05/2014; 155(1). DOI:10.1016/j.jep.2014.05.032 · 2.94 Impact Factor