Development of polymorphic microsatellite markers for Cymbidium goeringii (Orchidaceae)
Department of Biological Science, Kongju National University, Gongju, Chungnam 314-701, Korea.American Journal of Botany (Impact Factor: 2.6). 04/2012; 99(5):e193-8. DOI: 10.3732/ajb.1100505
• Premise of the study: Microsatellite markers were developed in Cymbidium goeringii to investigate its genetic diversity and population genetic structure. • Methods and Results: From a microsatellite-enriched genomic library, 21 novel polymorphic microsatellites were isolated. The polymorphic patterns were verified in four populations (East Korea, West Korea, China, and Japan). The number of alleles per locus ranged from 11 to 29 with a mean of 20.29. The observed and expected heterozygosities varied from 0.272 to 0.799 and from 0.461 to 0.911, respectively. • Conclusions: These microsatellite markers will be useful tools for understanding genetic variation and population ecogenetic structure in C. goeringii.
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ABSTRACT: Cymbidium goeringii is one of the most important and popular species in the orchid family in north-east Asia. In the present study, we prepared multiplex PCR system, and used it for the genotyping of eight simple sequence repeats (SSR) markers (CG409, CG415, CG709, CG722, CG787, CG1023, CG1210, and CG1281) in subject with 40 samples of cultivated varieties. All the analyzed samples showed different combined genotypes. The average combined power of discrimination was very high value of 7.14 x 10(-10), and observed heterozygosity (Ho = 0.466) was similar with two wild populations of C. goeringii, which may indicate no or little occurrence of genetic change after collection from wild habitats. The present study also developed a two-dimensional barcode to express information of genotype results of eight SSR markers (SSR DNA ID). The discrimination power of DNA ID between two individuals will be statistically more than 99.999999%. The SSR DNA ID and two-dimensional barcode may be very usefully applied for the discrimination and maintence of cultivars of C. goeringii.Wonye kwahak kisulchi = Korean journal of horticultural science and technology / 06/2012; 30(3):278-285. DOI:10.7235/hort.2012.12012 · 0.34 Impact Factor
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