Article

Development of polymorphic microsatellite markers for Cymbidium goeringii (Orchidaceae).

Department of Biological Science, Kongju National University, Gongju, Chungnam 314-701, Korea.
American Journal of Botany (impact factor: 2.66). 04/2012; 99(5):e193-8. DOI:10.3732/ajb.1100505 pp.e193-8
Source: PubMed

ABSTRACT • Premise of the study: Microsatellite markers were developed in Cymbidium goeringii to investigate its genetic diversity and population genetic structure. • Methods and Results: From a microsatellite-enriched genomic library, 21 novel polymorphic microsatellites were isolated. The polymorphic patterns were verified in four populations (East Korea, West Korea, China, and Japan). The number of alleles per locus ranged from 11 to 29 with a mean of 20.29. The observed and expected heterozygosities varied from 0.272 to 0.799 and from 0.461 to 0.911, respectively. • Conclusions: These microsatellite markers will be useful tools for understanding genetic variation and population ecogenetic structure in C. goeringii.

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Keywords

Cymbidium goeringii
 
genetic diversity
 
Japan
 
locus
 
microsatellite-enriched genomic library
 
population ecogenetic structure
 
population genetic structure
 
populations
 
understanding genetic variation
 
• Conclusions
 
• Methods
 
• Premise