The Antimicrobial Efficacy of Elaeis guineensis: Characterization, in Vitro and in Vivo Studies

Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Pulau Pinang 11800, Malaysia.
Molecules (Impact Factor: 2.42). 12/2012; 17(5):4860-77. DOI: 10.3390/molecules17054860
Source: PubMed


The urgent need to treat multi-drug resistant pathogenic microorganisms in chronically infected patients has given rise to the development of new antimicrobials from natural resources. We have tested Elaeis guineensis Jacq (Arecaceae) methanol extract against a variety of bacterial, fungal and yeast strains associated with infections. Our studies have demonstrated that E. guineensis exhibits excellent antimicrobial activity in vitro and in vivo against the bacterial and fungal strains tested. A marked inhibitory effect of the E. guineensis extracts was observed against C. albicans whereby E. guineensis extract at ½, 1, or 2 times the MIC significantly inhibited C. albicans growth with a noticeable drop in optical density (OD) of the bacterial culture. This finding confirmed the anticandidal activity of the extract on C. albicans. Imaging using scanning (SEM) and transmission (TEM) electron microscopy was done to determine the major alterations in the microstructure of the extract-treated C. albicans. The main abnormalities noted via SEM and TEM studies were the alteration in morphology of the yeast cells. In vivo antimicrobial activity was studies in mice that had been inoculated with C. albicans and exhibited good anticandidal activity. The authors conclude that the extract may be used as a candidate for the development of anticandidal agent.

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    • "The presence of flavonol, hydroxycinnamix acid, phenolic and o-Diphenol in oil palm leaf has also been reported by Ng & Choo (2010). Characterization of the oil palm leaf compound involved in biochemical and molecular defense against pathogen has been conducted by Vijayarathna et al. (2012) using FTIR analysis and demonstrated eight functional groups which were (OH) stretching vibrations from phenols present in the extract, carboxylic acid groups, aromatic, alkene C=C, primary alcohol and phenol groups. Therefore, there may be possibility of these compounds to be responsible for the antimicrobial against Ganoderma observed in the current study. "
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    BioMed Research International 08/2015; 2015(1). DOI:10.1155/2015/247382 · 2.71 Impact Factor
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    • "The growth curves were established by using the time and material consuming method of viable counts. Optical density (OD) is often preferred [17] but many bacterial strains (including Staphylococcus and Pseudomonas ones) form extracellular compounds increase the OD without changes in viable count. At MIC/2 and MIC/4, our growth curves show both a lag phase before reaching the exponential phase and a lower final concentration. "
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    Evidence-based Complementary and Alternative Medicine 11/2013; 2013(4):604536. DOI:10.1155/2013/604536 · 1.88 Impact Factor
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