Complete genome sequence of Mycobacterium intracellulare strain ATCC 13950(T).
ABSTRACT Here we report the first complete genome sequence of Mycobacterium intracellulare ATCC 13950(T), a Mycobacterium avium complex (MAC) strain. This genome sequence will serve as a valuable reference for understanding the epidemiologic, biological, and pathogenic aspects of the disparity between MAC members.
- SourceAvailable from: Bum-Joon Kim[Show abstract] [Hide abstract]
ABSTRACT: Here, we report the draft genome sequence of the clinical strain MOTT-H4Y, grouped previously into the INT5 genotype of the 5 genotypes of .Genome Announcements 01/2013; 1(1).
- [Show abstract] [Hide abstract]
ABSTRACT: Here, we report the complete genome sequence of Mycobacterium yongonense DSM 45126(T), genetically closely related to the INT5 genotype of M. intracellulare.Genome Announcements 06/2013; 1(4).
- [Show abstract] [Hide abstract]
ABSTRACT: Mycobacteria comprise diverse species including non-pathogenic, environmental organisms, animal disease agents and human pathogens, notably Mycobacterium tuberculosis. Considering that the mycobacterial cell wall constitutes a significant barrier to drug penetration, the aim of this study was to conduct a comparative genomics analysis of the repertoire of enzymes involved in peptidoglycan (PG) remodelling to determine the potential of exploiting this area of bacterial metabolism for the discovery of new drug targets. We conducted an in silico analysis of 19 mycobacterial species/clinical strains for the presence of genes encoding resuscitation promoting factors (Rpfs), penicillin binding proteins, endopeptidases, L,D-transpeptidases and N-acetylmuramoyl-L-alanine amidases. Our analysis reveals extensive genetic multiplicity, allowing for classification of mycobacterial species into three main categories, primarily based on their rpf gene complement. These include the M. tuberculosis Complex (MTBC), other pathogenic mycobacteria and environmental species. The complement of these genes within the MTBC and other mycobacterial pathogens is highly conserved. In contrast, environmental strains display significant genetic expansion in most of these gene families. Mycobacterium leprae retains more than one functional gene from each enzyme family, underscoring the importance of genetic multiplicity for PG remodelling. Notably, the highest degree of conservation is observed for N-acetylmuramoyl-L-alanine amidases suggesting that these enzymes are essential for growth and survival. PG remodelling enzymes in a range of mycobacterial species are associated with extensive genetic multiplicity, suggesting functional diversification within these families of enzymes to allow organisms to adapt.BMC Microbiology 03/2014; 14(1):75. · 2.98 Impact Factor
Complete Genome Sequence of Mycobacterium intracellulare Strain
Byoung-Jun Kim,aBeom-Soon Choi,bJong-Sung Lim,bIk-Young Choi,bJe-Hee Lee,cJongsik Chun,c,dYoon-Hoh Kook,a
and Bum-Joon Kima
Department of Microbiology and Immunology, Liver Research Institute, Cancer Research Institute and SNUMRC, College of Medicine, Seoul National University, Seoul,
Republic of Koreaa; National Instrumentation Center for Environmental Management, Seoul National University, Seoul, Republic of Koreab; Chunlab, Inc., Seoul National
University, Seoul, Republic of Koreac; and School of Biological Sciences, Seoul National University, Seoul, Republic of Koread
Here we report the first complete genome sequence of Mycobacterium intracellulare ATCC 13950T, a Mycobacterium avium
(MAC) are the nontuberculous mycobacteria most frequently iso-
lated in clinical settings (5–7). Traditionally, the MAC includes two
species, M. avium and Mycobacterium intracellulare (1, 3, 6). There
epidemiologic, biological, and pathogenic aspects. Currently, of the
biense (AFVW00000000) (2), closely related to M. avium, are avail-
able. However, the complete genome sequence of M. intracellulare
has not yet been determined. To better understand the pathogenic
mechanism of M. intracellulare, we report the complete, annotated
genome sequence of M. intracellulare ATCC 13950Tin the present
The M. intracellulare genome was sequenced by a standard shot-
gun strategy using GS FLX pyrosequencing technology. Sequencing
analysis was performed at the National Instrumentation Center for
Environmental Management (Genome Analysis Unit) at Seoul Na-
tional University. A total of 921,179 reads were generated, with an
average read length of 400, yielding a total sequence of 368,366,484
bp. This represents 68? coverage of the estimated 5.4-Mb genome.
genome sequences of reference strain using the BLASTZ program
(http://www.psc.edu/general/software/packages/blastz/). All of the
remaining gaps between contigs were completely filled by ?50-fold
Solexa reads and PCR amplifications. Genome annotation was per-
formed using the NCBI Prokaryotic Genomes Automatic Annota-
Our data on the M. intracellulare genome show it to have a
circular DNA of 5,402,402 bp, which is larger than the genome of
M. avium subsp. paratuberculosis (4.8 Mb) and contains more
protein coding genes (5,145 versus 4,400) and more tRNA genes
a G?C content of 68.10%, and no plasmid was found. M. intra-
cellulare is known to form a close cluster with M. avium in a phy-
logenetic analysis based on the 16S rRNA gene sequence. Our
the NCBI microbial sequence database also supported the close
relationships of M. intracellulare with M avium subsp. avium 104
and M. avium subsp. paratuberculosis. The genome sequence re-
epidemiologic, biological, and pathogenic aspects of the disparity
between MAC members.
Nucleotide sequence accession number. The whole-genome
sequence of M. intracellulare ATCC 13950Thas been deposited in
the GenBank database under accession number CP003322.
This work was supported by a National Research Foundation of Korea
grant funded by the Korean Government Ministry of Education, Science
and Technology (2010-0014269).
1. Falkinham JO, III. 1996. Epidemiology of infection by nontuberculous
mycobacteria. Clin. Microbiol. Rev. 9:177–215.
2. González-Pérez M, Murcia MI, Landsman D, Jordan IK, Marino-
strain, CECT 3035. J. Bacteriol. 193:5866–5867.
3. Inderlied CB, Kemper CA, Bermudez LE. 1993. The Mycobacterium
avium complex. Clin. Microbiol. Rev. 6:266–310.
4. Li L, et al. 2005. The complete genome sequence of Mycobacterium avium
subspecies paratuberculosis. Proc. Natl. Acad. Sci. U. S. A. 102:12344–
5. Tortoli E, et al. 1999. Use of BACTEC MGIT 960 for recovery of myco-
bacteria from clinical specimens: multicenter study. J. Clin. Microbiol. 37:
6. Turenne CY, Wallace R, Jr, Behr MA. 2007. Mycobacterium avium in the
postgenomic era. Clin. Microbiol. Rev. 20:205–229.
7. Yajko DM, et al. 1995. Mycobacterium avium complex in water, food, and
Acquir. Immune. Defic. Syndr. Hum. Retrovirol. 9:176–182.
Received 22 February 2012 Accepted 2 March 2012
Address correspondence to Bum-Joon Kim, email@example.com.
Copyright © 2012, American Society for Microbiology. All Rights Reserved.
jb.asm.org0021-9193/12/$12.00Journal of Bacteriologyp. 2750