Complete Genome Sequence of Mycobacterium intracellulare Strain ATCC 13950T
ABSTRACT Here we report the first complete genome sequence of Mycobacterium intracellulare ATCC 13950(T), a Mycobacterium avium complex (MAC) strain. This genome sequence will serve as a valuable reference for understanding the epidemiologic, biological, and pathogenic aspects of the disparity between MAC members.
Full-textDOI: · Available from: Bum-Joon Kim, Sep 27, 2015
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- "However, because of the unique microbiological nature of Mycobacterium, such as slow growth and difficulty involved in performing cell wall lysis owing to its unique cell wall structure, polymerase chain reaction (PCR)-based molecular typing methods are preferred than PFGE, although the latter is considered a “gold standard” molecular typing method in other bacterial species. The recent identification of complete genome sequence of M. intracellulare made it possible to develop effective VNTR methods [11,13]. "
Article: Molecular Typing of Mycobacterium intracellulare Using Pulsed-Field Gel Electrophoresis, Variable-Number Tandem-Repeat Analysis, Mycobacteria Interspersed Repetitive-Unit-Variable-Number Tandem Repeat Typing, and Multilocus Sequence Typing: Molecular Characterization and Comparison of Each Typing Methods[Show abstract] [Hide abstract]
ABSTRACT: Objectives: Mycobacterium intracellulare is the major causative agent of nontuberculous mycobacteria-related pulmonary infections. The strain typing of M. intracellulare is important for the treatment and control of its infections. We compared the discrimination capacity and effective value of four different molecular typing methods. Methods: Antibiotic susceptibility testing, hsp65 and rpoB sequencing, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), mycobacteria interspersed repetitive-unit-variable-number tandem-repeat analysis (MIRU-VNTR), and VNTR assay targeting 44 M. intracellulare isolates obtained from patients with pulmonary infections were performed. Results: All the antibiotic susceptibility patterns had no association with the molecular and sequence types tested in this study; however, the molecular and sequence types were related with each other. PFGE gave best results for discriminatory capacity, followed by VNTR, MLST, and MIRU-VNTR. Conclusion: The high discriminatory power of PFGE, VNTR, and MLST is enough for differentiating between reinfection and relapse, as well as for other molecular epidemiological usages. The MLST could be regarded as a representative classification method, because it showed the clearest relation with the sequence types.06/2014; 5(3):119-30. DOI:10.1016/j.phrp.2014.04.003
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ABSTRACT: Here, we report the first complete genome sequence of the Mycobacterium intracellulare clinical strain MOTT-02, which was previously grouped in the INT2 genotype of M. intracellulare. This genome sequence will serve as a valuable reference for improving the understanding of the disparity in the virulence and epidemiologic traits between M. intracellulare genotypes.Journal of bacteriology 05/2012; 194(10):2771. DOI:10.1128/JB.00365-12 · 2.81 Impact Factor
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ABSTRACT: Here, we report the complete genome sequence of the Mycobacterium intracellulare clinical strain MOTT-64, previously grouped into the INT1 genotype among five genotypes of M. intracellulare. This genome sequence will serve as a valuable reference for understanding the disparity in the virulence and epidemiologic traits among M. intracellulare genotypes.Journal of bacteriology 06/2012; 194(12):3268. DOI:10.1128/JB.00471-12 · 2.81 Impact Factor