Micromonospora sediminicola sp. nov., isolated from a marine sediment of the Andaman Sea of Thailand.
ABSTRACT An actinomycete strain, SH2-13T, was isolated from a marine sediment sample collected from the Andaman Sea of Thailand. Applying a polyphasic approach, the isolate was identified as a member of the genus Micromonospora using morphological and chemotaxonomic characteristics, including the presence of meso-diaminopimelic acid in the peptidoglycan, whole cell sugars were arabinose, galactose, glucose, rhamnose, ribose and xylose. Diagnostic polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, phosphoglycolipids. The major menaquinones were MK-10(H2), MK-10(H4) and MK-10(H6). The 16S rRNA gene sequence analysis revealed the similarity to Micromonospora marina JSM1-1T (99.1 %), Micromonospora coxensis 2-30-b/28T (99.1 %), Micromonospora aurantiaca DSM 43813T (98.8 %) and Micromonospora chalcea DSM 43026T (98.7 %). However, a combination of DNA-DNA hybridization results and phenotypic properties indicated that strain SH2-13T (=NBRC 107934T =BCC 45601T) should be classified as the type strain of a novel species, Micromonospora sediminicola sp. nov. is proposed.