Diversity of Cellulolytic Microbes and the Biodegradation of Municipal Solid Waste by a Potential Strain

Central Pollution Control Board, New Delhi, India.
International Journal of Microbiology 02/2012; 2012:325907. DOI: 10.1155/2012/325907
Source: PubMed


Municipal solid waste contains high amounts of cellulose, which is an ideal organic waste for the growth of most of microorganism as well as composting by potential microbes. In the present study, Congo red test was performed for screening of microorganism, and, after selecting a potential strains, it was further used for biodegradation of organic municipal solid waste. Forty nine out of the 250 different microbes tested (165 belong to fungi and 85 to bacteria) produced cellulase enzyme and among these Trichoderma viride was found to be a potential strain in the secondary screening. During the biodegradation of organic waste, after 60 days, the average weight losses were 20.10% in the plates and 33.35% in the piles. There was an increase in pH until 20 days. pH however, stabilized after 30 days in the piles. Temperature also stabilized as the composting process progressed in the piles. The high temperature continued until 30 days of decomposition, after which the temperature dropped to 40°C and below during the maturation. Good quality compost was obtained in 60 days.

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Available from: Mukesh Awasthi, Jan 28, 2015
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    • "Mucor sp. was not isolated from saw dust samples. This finding is in line with previously reported studies [22–26] that members of the genera Aspergillus and Trichoderma were the dominant fungi in forest and agricultural soils. Fungi have many different functions in soils, which include either active roles, such as the degradation of dead plant material, or inactive roles where propagules are present in the soil as a resting stage [27]. "
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    ABSTRACT: The aim of this work is to select filamentous fungal strains isolated from saw dust, soil, and decaying wood with the potential to produce xylanase and cellulase enzymes. A total of 110 fungi were isolated. Fifty-seven (57) of these fungi were isolated from soil samples, 32 from sawdust, and 19 from decaying wood. Trichoderma and Aspergillus had the highest relative occurrence of 42.6% and 40.8%, respectively. Trichoderma viride Fd18 showed the highest specific activity of 1.30 U mg(-1) protein for xylanase, while the highest cellulase activity of 1.23 U mg(-1) was shown by Trichoderma sp. F4. The isolated fungi demonstrated potential for synthesizing the hydrolytic enzymes.
    12/2013; 2013:283423. DOI:10.1155/2013/283423
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    ABSTRACT: Aims of the Study: The aim of this study was to determine the amount of lignin degradation and biodegradation of organic matter and change of biomass under compost and vermicomposting of sewage sludge. Materials & Methods: Sawdust was added to sewage sludge at 1:3 weight bases to Carbon to Nitrogen ratio of 25:1 for composting or vermicomposting. Lignin and volatile solids were determined at different periods, of 0, 10, 30, 40 and 60 days of composting or vermicomposting period to determine the biodegradation of lignocellulose to lignin. Results were expressed as mean of two replicates and the comparisons among means were made using the least significant difference test calculated (p <0.05). Results: After 60 days of experiment period, the initial lignin increased from 3.46% to 4.48% for compost and 3.46% to 5.27% for vermicompost. Biodegradation of lignocellulose was very slow in compost and vermicompost processes. Vermicomposting is a much faster process than compost to convert lignocellulose to lignin (p <0.05). Conclusions: The organic matter losses in sewage sludge composting and vermicomposting are due to the degradation of the lignin fractions. By increasing compost age, the amount of volatile solids will decrease.
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    ABSTRACT: The use of polythene is increasing day by day and its degradation is becoming a great challenge. Annually about 500 billion to 1 trillion polythene carry bags are being consumed around the globe. Polythene is durable and needs up to 1000 years for natural degradation in the environment. In the present review, an attempt has been made to pool all the available literature on the biodegradation of polythene under the following objectives: (1) to highlight the level of polythene pollution; (2) to enlist the cost effective methods; (3) to pool the source of polythene degrading microbes; (4) to brief the mechanism of polythene degradation; (5) to highlight the methods used for the biodegradation of the polythene; (6) to discuss the assessment of polythene degradation by efficient microbes; (7) to enlist the products of polythene under degradation process; (8) to test the toxicity level of the products of the degraded polythene, and (9) to discuss the future aspects of polythene degradation.
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