Cerium oxide-chitosan based nanobiocomposite for food borne mycotoxin detection

Applied Physics Letters (Impact Factor: 3.3). 11/2009; 95(17):173703 - 173703-3. DOI: 10.1063/1.3249586
Source: IEEE Xplore


Cerium oxide nanoparticles ( NanoCeO 2) and chitosan (CH) based nanobiocomposite film deposited onto indium-tin-oxide coated glass substrate has been used to coimmobilize rabbit immunoglobin (r-IgGs) and bovine serum albumin (BSA) for food borne mycotoxin [ochratoxin-A (OTA)] detection. Electrochemical studies reveal that presence of NanoCeO 2 increases effective electro-active surface area of CH-NanoCeO 2/ indium tin oxide (ITO) nanobiocomposite resulting in high loading of r-IgGs. BSA / r-IgGs / CH-NanoCeO 2/ ITO immunoelectrode exhibits improved linearity (0.25–6.0 ng/dl), detection limit (0.25 ng/dl), response time (25 s), sensitivity (18 μ A / ng   dl -1  cm -2) , and regression coefficient ( r 2∼0.997) .

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