Cerium oxide-chitosan based nanobiocomposite for food borne mycotoxin detection

Applied Physics Letters (Impact Factor: 3.52). 11/2009; DOI: 10.1063/1.3249586
Source: IEEE Xplore

ABSTRACT Cerium oxide nanoparticles ( NanoCeO 2) and chitosan (CH) based nanobiocomposite film deposited onto indium-tin-oxide coated glass substrate has been used to coimmobilize rabbit immunoglobin (r-IgGs) and bovine serum albumin (BSA) for food borne mycotoxin [ochratoxin-A (OTA)] detection. Electrochemical studies reveal that presence of NanoCeO 2 increases effective electro-active surface area of CH-NanoCeO 2/ indium tin oxide (ITO) nanobiocomposite resulting in high loading of r-IgGs. BSA / r-IgGs / CH-NanoCeO 2/ ITO immunoelectrode exhibits improved linearity (0.25–6.0 ng/dl), detection limit (0.25 ng/dl), response time (25 s), sensitivity (18 μ A / ng   dl -1  cm -2) , and regression coefficient ( r 2∼0.997) .

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    ABSTRACT: Ochratoxin-A [7-(L-β-phenylalanylcarbonyl)-carboxyl-5-chloro-8-hydroxy-3,4-dihydro-3R-methyl-isocumarin, OTA] is a common food contaminant mycotoxin that enters the human body through the consumption of improperly stored food products. Upon ingestion, it leads to immuno-suppression and immuno-toxicity. OTA has been known to produce nephrotoxic, teratogenic, and carcinogenic activity (via oxidative DNA damage) in several species. This review intro-duces potentials of electrochemical biosensor to provide breakthroughs in OTA detection through improved selectivity and sensitivity and also the current approaches for detecting OTA in food products.
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    ABSTRACT: An electrochemical biosensor based on manganese oxide (Mn3O4) and chitosan (Cn) nanocomposite has been fabricated for fish freshness detection. The electrophoretic deposition of Mn3O4 nanoparticles (15–20 nm) with Cn has changed their morphological arrangement leading to pearl shaped of Mn3O4–Cn nanocomposite on indium tin oxide substrate. Size and morphology of nanocomposite have been confirmed by high resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD) and scanning electron microscopy (SEM). The results of electrochemical response reveal that this improved sensor has widest detection range of xanthine concentration from 1 to 500 µM and excellent sensitivity of 1.46 μA µM−1 cm−2. The fabricated XOx/Mn3O4–Cn/ITO biosensor can detect as low as 1.31 μM of xanthine and lower Km value of 0.018 μM confirming its superior affinity towards the nanocomposite film.
    Biosensors & Bioelectronics 06/2014; · 6.45 Impact Factor
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    ABSTRACT: We report results of studies relating to the development of an electrochemical immunosensor based on carboxylated multiwalled carbon nanotubes (c-MWCNTs) electrophoretically deposited onto indium tin oxide (ITO) glass. This c-MWCNTs/ITO electrode surface has been functionalized with monoclonal aflatoxin B1 antibodies (anti-AFB1) for the detection of aflatoxin-B1 using electrochemical technique. Electron microscopy, X-ray diffraction and Raman studies suggest the successful synthesis of c-MWCNTs and the Fourier transform infra-red spectroscopic (FT-IR) studies reveal its carboxylic functionalized nature. The proposed immunosensor shows high sensitivity (95.2 μA ng−1mL cm−2), improved detection limit (0.08 ng mL−1) in the linear detection range of 0.25-1.375 ng mL−1. The low value of association constant (0.0915 ng mL−1) indicates high affinity of immunoelectrode towards aflatoxin (AFB1).
    Sensors and Actuators B Chemical 04/2013; · 3.84 Impact Factor


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May 28, 2014