Cerium oxide-chitosan based nanobiocomposite for food borne mycotoxin detection

Applied Physics Letters (Impact Factor: 3.52). 11/2009; 95(17):173703 - 173703-3. DOI: 10.1063/1.3249586
Source: IEEE Xplore

ABSTRACT Cerium oxide nanoparticles ( NanoCeO 2) and chitosan (CH) based nanobiocomposite film deposited onto indium-tin-oxide coated glass substrate has been used to coimmobilize rabbit immunoglobin (r-IgGs) and bovine serum albumin (BSA) for food borne mycotoxin [ochratoxin-A (OTA)] detection. Electrochemical studies reveal that presence of NanoCeO 2 increases effective electro-active surface area of CH-NanoCeO 2/ indium tin oxide (ITO) nanobiocomposite resulting in high loading of r-IgGs. BSA / r-IgGs / CH-NanoCeO 2/ ITO immunoelectrode exhibits improved linearity (0.25–6.0 ng/dl), detection limit (0.25 ng/dl), response time (25 s), sensitivity (18 μ A / ng   dl -1  cm -2) , and regression coefficient ( r 2∼0.997) .

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    ABSTRACT: An electrochemical biosensor based on manganese oxide (Mn3O4) and chitosan (Cn) nanocomposite has been fabricated for fish freshness detection. The electrophoretic deposition of Mn3O4 nanoparticles (15–20 nm) with Cn has changed their morphological arrangement leading to pearl shaped of Mn3O4–Cn nanocomposite on indium tin oxide substrate. Size and morphology of nanocomposite have been confirmed by high resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD) and scanning electron microscopy (SEM). The results of electrochemical response reveal that this improved sensor has widest detection range of xanthine concentration from 1 to 500 µM and excellent sensitivity of 1.46 μA µM−1 cm−2. The fabricated XOx/Mn3O4–Cn/ITO biosensor can detect as low as 1.31 μM of xanthine and lower Km value of 0.018 μM confirming its superior affinity towards the nanocomposite film.
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May 28, 2014