Cerium oxide-chitosan based nanobiocomposite for food borne mycotoxin detection

Applied Physics Letters (Impact Factor: 3.52). 11/2009; DOI: 10.1063/1.3249586
Source: IEEE Xplore

ABSTRACT Cerium oxide nanoparticles ( NanoCeO 2) and chitosan (CH) based nanobiocomposite film deposited onto indium-tin-oxide coated glass substrate has been used to coimmobilize rabbit immunoglobin (r-IgGs) and bovine serum albumin (BSA) for food borne mycotoxin [ochratoxin-A (OTA)] detection. Electrochemical studies reveal that presence of NanoCeO 2 increases effective electro-active surface area of CH-NanoCeO 2/ indium tin oxide (ITO) nanobiocomposite resulting in high loading of r-IgGs. BSA / r-IgGs / CH-NanoCeO 2/ ITO immunoelectrode exhibits improved linearity (0.25–6.0 ng/dl), detection limit (0.25 ng/dl), response time (25 s), sensitivity (18 μ A / ng   dl -1  cm -2) , and regression coefficient ( r 2∼0.997) .

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    ABSTRACT: Ochratoxin-A [7-(L-β-phenylalanylcarbonyl)-carboxyl-5-chloro-8-hydroxy-3,4-dihydro-3R-methyl-isocumarin, OTA] is a common food contaminant mycotoxin that enters the human body through the consumption of improperly stored food products. Upon ingestion, it leads to immuno-suppression and immuno-toxicity. OTA has been known to produce nephrotoxic, teratogenic, and carcinogenic activity (via oxidative DNA damage) in several species. This review intro-duces potentials of electrochemical biosensor to provide breakthroughs in OTA detection through improved selectivity and sensitivity and also the current approaches for detecting OTA in food products.
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    ABSTRACT: Nanostructured metal oxides (NMOs) have recently become important as materials that provide an effective surface for biomolecule immobilization with desired orientation, better conformation and high biological activity resulting in enhanced sensing characteristics. Nanostructured metal oxides with unique optical, electrical and molecular properties along with desired functionalities and surface charge properties provide interesting platforms for interfacing biorecognition elements with transducers for signal amplification. In this review, we discuss the various approaches that have been adopted for improving the performance of NMO-based biosensors for clinical and non-clinical applications. The performance of an NMO-based biosensor can be improved by tailoring the properties of the metal oxide–biomolecule interface through engineering of morphology, particle size, effective surface area, functionality, adsorption capability and electron-transfer properties. These interesting NMOs are expected to find applications in a new generation of miniaturized, smart biosensing devices.
    NPG Asia Materials. 12/2010; 3(1):17-24.
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    ABSTRACT: We report results of studies relating to the development of an electrochemical immunosensor based on carboxylated multiwalled carbon nanotubes (c-MWCNTs) electrophoretically deposited onto indium tin oxide (ITO) glass. This c-MWCNTs/ITO electrode surface has been functionalized with monoclonal aflatoxin B1 antibodies (anti-AFB1) for the detection of aflatoxin-B1 using electrochemical technique. Electron microscopy, X-ray diffraction and Raman studies suggest the successful synthesis of c-MWCNTs and the Fourier transform infra-red spectroscopic (FT-IR) studies reveal its carboxylic functionalized nature. The proposed immunosensor shows high sensitivity (95.2 μA ng−1mL cm−2), improved detection limit (0.08 ng mL−1) in the linear detection range of 0.25-1.375 ng mL−1. The low value of association constant (0.0915 ng mL−1) indicates high affinity of immunoelectrode towards aflatoxin (AFB1).
    Sensors and Actuators B Chemical 04/2013; · 3.84 Impact Factor


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May 28, 2014