Chemical surface modification of poly-ε-caprolactone improves Schwann cell proliferation for peripheral nerve repair
ABSTRACT Poly-ε-caprolactone (PCL) is a biodegradable and biocompatible polymer used in tissue engineering for various clinical applications. Schwann cells (SCs) play an important role in nerve regeneration and repair. SCs attach and proliferate on PCL films but cellular responses are weak due to the hydrophobicity and neutrality of PCL. In this study, PCL films were hydrolysed and aminolysed to modify the surface with different functional groups and improve hydrophilicity. Hydrolysed films showed a significant increase in hydrophilicity while maintaining surface topography. A significant decrease in mechanical properties was also observed in the case of aminolysis. In vitro tests with Schwann cells (SCs) were performed to assess film biocompatibility. A short-time experiment showed improved cell attachment on modified films, in particular when amino groups were present on the material surface. Cell proliferation significantly increased when both treatments were performed, indicating that surface treatments are necessary for SC response. It was also demonstrated that cell morphology was influenced by physico-chemical surface properties. PCL can be used to make artificial conduits and chemical modification of the inner lumen improves biocompatibility Copyright © 2012 John Wiley & Sons, Ltd.
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ABSTRACT: Peripheral nerve injuries represent a substantial clinical problem with insufficient or unsatisfactory treatment options. This review summarises all the events occurring after nerve damage at the level of the cell body, the site of injury and the target organ. Various experimental strategies to improve neuronal survival, axonal regeneration and target reinnervation are described including pharmacological approaches and cell-based therapies. Given the complexity of nerve regeneration, further studies are needed to address the biology of nerve injury, to improve the interaction with implantable scaffolds, and to implement cell-based therapies in nerve tissue engineering.Advanced Drug Delivery Reviews 11/2014; DOI:10.1016/j.addr.2014.11.010 · 12.71 Impact Factor
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ABSTRACT: Electrospun fibrous membranes coated with basic fibroblast growth factor (bFGF) are effective medical devices to promote wound healing. However, the current strategies of adding bFGF generally cause degradation of electrospun materials or damage to the bioactivity of the biomolecules. Here, we have developed a simple strategy for surface bFGF-functionalization of electrospun fibers in an aqueous solution, which maintained original fiber properties and growth factor bioactivity. Polydopamine (PDA) forming the mussel foot protein was chosen as an adhesive polymeric bridge-layer between substrate poly(lactide-co-glycolide) (PLGA) fibers and bFGF. The bFGF-grafted PDA was analyzed using scanning electron microscopy, water contact angle measurements, and X-ray photoelectron spectroscopy. Improved hydrophilicity together with a stable fibrous structure and biodegradable fibrous matrix suggested that the PLGA/PDA-bFGF electrospun fibrous scaffolds have great potential for promoting wound healing. In vitro experiments showed that the bFGF-grafted PLGA electrospun fibrous scaffolds have highly enhanced adhesion, viability, and proliferation of human dermal fibroblasts. In vivo results showed that such scaffolds shortened wound healing time, accelerated epithelialization and promoted skin remodeling. Therefore, this PDA modification method can be a useful tool to graft biomolecules onto polymeric electrospun fibrous scaffolds which are potential scaffold candidates for repairing skin tissue.05/2014; 2(23). DOI:10.1039/C3TB21814G
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ABSTRACT: Studies have explored many approaches to prevent and treat hypertrophic scars. However, most of them inhibit hypertrophic scars after their formation, without taking into account repairing tissue damage in the early stage and inhibiting scar hyperplasia in the late stage through combining treatments. In this study, Ginsenoside Rg3 (Rg3) loaded poly(D,L-lactide-co-glycolide) (PLGA) electrospun fibrous scaffolds were prepared by a co-solvent electrospinning method, and then hyaluronic acid (HA) was coated on the surface of the drug-loaded electrospun fibers by a pressure-driven permeation (PDP) wrapped method. The hydrophilic Rg3/PLGA/HA electrospun fibrous scaffolds showed the effect of combining treatments of promoting wound healing in the early stage and inhibiting scar hyperplasia in the late stage. The improved hydrophilicity together with a proper porous structure, a stable fibrous structure, durable mechanical properties and a similar drug release model suggested that the Rg3/PLGA electrospun scaffold coated with HA via PDP has great potential for drug-loaded tissue engineering scaffolds. The in vivo animal results showed that the Rg3/PLGA/HA could promote wound healing earlier and significantly inhibit scar hyperplasia compared to other control groups from macroscopic, histologic evaluation, and expression of collagen type I. The Rg3/PLGA/HA electrospun fibrous scaffolds open a new combined therapeutic approach for inhibiting hypertrophic scars.08/2013; 1(35). DOI:10.1039/C3TB20441C