Akt or phosphoinositide-3-kinase inhibition reverses cardio-protection in Toll-like receptor 2 deficient mice.
ABSTRACT Absence or inhibition of Toll-like receptor 2 (TLR2) signalling during murine myocardial ischaemia/reperfusion (MI/R) decreases myocardial necrosis and inflammation, thereby ameliorating cardiac dysfunction and improving survival. In the present study, we provide evidence for the involvement of the phosphoinositide-3-kinase/Akt pathway in TLR2-dependent reperfusion injury.
Adult male wild-type (WT) and TLR2(-/-) mice were subjected to myocardial ischaemia (30min) and reperfusion (4h). Animals were treated with phosphoinositide-3-kinase inhibitor wortmannin, Akt inhibitor V (triciribine), or vehicle 1h prior to MI/R. Protein expression levels of Akt1 and phosphoinositide-3-kinase and their respective phosphorylated forms were determined by Western blot analysis. Myocardial necrosis was quantified after staining with the tetrazolium method and by troponin T plasma levels.
TLR2(-/-) mice displayed significantly increased Akt and phospho-Akt levels compared to WT mice, whilst no significant difference in phosphoinositide-3-kinase expression and phosphorylation could be observed. TLR2(-/-) mice also showed a blunted myocardial necrosis, the extent of which inversely correlated with Akt expression and degree of phosphorylation. Pharmacological inhibition of both, phosphoinositide-3-kinase or Akt, reversed the cardioprotection observed in TLR2(-/-) mice, whilst no effect could be observed in WT mice.
Akt is an important mediator of cardioprotection in TLR2(-/-) animals during MI/R. The effect is, however, likely mediated by its genomic overexpression in the heart of TLR2(-/-) animals whilst Akt activation by phosphoinositide-3-kinase is unaltered.