Article

Vitamin C-silver: an environmentally benign choice for DNA visualization on polyacrylamide gels.

Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College, Wenzhou, P. R. China.
Analytical Sciences (impact factor: 1.25). 01/2012; 28(4):379-84. pp.379-84
Source: PubMed

ABSTRACT A practical, sensitive and environment-benign protocol for the detection of DNA on polyacrylamide gels was described. In this method, the most commonly used formaldehyde-based developer in DNA silver stain, which poses potential hazards to the health of operators, is firstly replaced by vitamin C (Vc) in sodium thiosulfate solution. This allows user-friendly and efficient visualization of DNA that takes about 20 min to complete all the procedures, and provides comparable sensitivity (8 pg of single band) to the most sensitive formaldehyde-based silver staining method developed before.

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Keywords

allows user-friendly
 
comparable sensitivity
 
complete
 
detection
 
DNA silver stain
 
efficient visualization
 
environment-benign protocol
 
formaldehyde-based developer
 
polyacrylamide gels
 
poses potential hazards
 
practical
 
procedures
 
sensitive formaldehyde-based silver staining method
 
single band
 
sodium thiosulfate solution
 
Vc
 
vitamin C
 

Zhongxin Zhu