Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide
Centre for Structural Biology & Division of Molecular Biosciences, Imperial College London, South Kensington, London SW7 2AZ, UK.Biochemical and Biophysical Research Communications (Impact Factor: 2.3). 04/2012; 421(2):208-13. DOI: 10.1016/j.bbrc.2012.03.136
The use of heavy water (D(2)O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D(2)O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD.
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