Genome Sequence of Janthinobacterium sp. Strain PAMC 25724,
Isolated from Alpine Glacier Cryoconite
Su Jin Kim,aSeung Chul Shin,bSoon Gyu Hong,bYung Mi Lee,bHyoungseok Lee,bJungeun Lee,bIn-Geol Choi,aand Hyun Parkb
College of Life Sciences and Biotechnology, Korea University, Seongbuk-gu, Seoul, South Korea,aand Korea Polar Research Institute, Yeonsu-gu, Incheon, South Koreab
Thedraftgenomeof Janthinobacterium sp.strainPAMC25724,whichisaviolacein-producingpsychrotolerantbacterium,was
monly isolated from the microbiota of soils and water of rivers,
soil (4, 7). Janthinobacterium sp. is one of the most common pur-
violacein (6), and it is suggested that the inhibitory effects of the
PAMC 25724 was isolated from glacier cryoconite of the Alps
mountain range, Austria (47°04=N, 12°41=E).
The genome of Janthinobacterium sp. PAMC 25724 was ana-
lyzed using a combined approach with the 454 GS FLX Titanium
system (Roche Diagnostics, Branford, CT) with an 8-kb paired-
end library (111,434 reads) and the Illumina GAIIx (San Diego,
GS FLX sequencing achieved about 8.7-fold coverage, while 208-
fold read coverage was achieved by Illumina paired-end sequenc-
ing. The reads generated by the Illumina GAIIx and the 454 GS
FLX Titanium were assembled using Celera assembler 6.1 (5).
(3), the rapid annotations using subsystems technology (RAST)
server (1), and the NCBI COG database (8). The draft genome se-
quence of Janthinobacterium sp. PAMC 25724 includes 4,985,247
5S rRNA genes, one 23S rRNA gene, and one 16S rRNA gene were
predicted in the draft genome. Approximately 84.9% of nucleotides
were predicted as protein-coding regions, and 73.7% (3,197) of the
Janthinobacterium sp. Marseille (score, 537), Herminiimonas arseni-
coxydans (score, 422), and Oxalobacter formigenes OXCC13 (score,
he genus Janthinobacterium includes violacein-producing,
Gram-negative, motile, rod-shaped bacteria that are com-
in this paper is the first version, AHHB01000000.
This work was supported by a Functional Genomics on Polar Organisms
grant (PE12020) funded by the Korea Polar Research Institute (KOPRI).
technology. BMC Genomics 9:75.
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Received 19 January 2012 Accepted 2 February 2012
Address correspondence to Hyun Park, firstname.lastname@example.org, or In-Geol Choi,
S.J.K. and S.C.S. contributed equally to this publication.
Copyright © 2012, American Society for Microbiology. All Rights Reserved.
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