Genome Sequence of Janthinobacterium sp. Strain PAMC 25724, Isolated from Alpine Glacier Cryoconite
ABSTRACT The draft genome of Janthinobacterium sp. strain PAMC 25724, which is a violacein-producing psychrotolerant bacterium, was determined. The strain was isolated from glacier cryoconite of the Alps mountain permafrost region. The sequence will allow identification and characterization of the genetic determination of its cold-adaptive properties.
Full-textDOI: · Available from: Hyoungseok Lee, Feb 12, 2014
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Article: Genome Sequence of Janthinobacterium sp. Strain PAMC 25724, Isolated from Alpine Glacier Cryoconite
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- "We contend there is a paucity of information on the properties of cryoconite organic matter and microbial community structure from mountain glaciers. In particular, studies of European alpine cryoconite diversity have hitherto been limited to culture-dependent studies (Margesin et al., 2002; Kim et al., 2012). Recently, Edwards et al. (2013b) presented a 1.2 Gbp metagenome assembly from pooled cryoconite sampled on Rotmoosferner in the Austrian Alps. "
ABSTRACT: Cryoconite holes are known as foci of microbial diversity and activity on polar glacier surfaces, but are virtually unexplored microbial habitats in alpine regions. In addition, whether cryoconite community structure reflects ecosystem functionality is poorly understood. Terminal-Restriction Fragment Length Polymorphism and Fourier Transform -Infra Red metabolite fingerprinting of cryoconite from glaciers in Austria, Greenland and Svalbard demonstrated cryoconite bacterial communities are closely correlated with cognate metabolite fingerprints. The influence of bacterial-associated fatty acids and polysaccharides was inferred, underlining the importance of bacterial community structure in the properties of cryoconite. Thus, combined application of T-RFLP and FT-IR metabolite fingerprinting promise high throughput and hence, rapid assessment of community structure-function relationships. Pyrosequencing revealed Proteobacteria were particularly abundant, with Cyanobacteria likely acting as ecosystem engineers in both alpine and Arctic cryoconite communities. However, despite these generalities, significant differences in bacterial community structures, compositions and metabolomes are found between alpine and Arctic cryoconite habitats, reflecting the impact of local and regional conditions on the challenges of thriving in glacial ecosystems. This article is protected by copyright. All rights reserved.FEMS Microbiology Ecology 01/2014; 89(2). DOI:10.1111/1574-6941.12283 · 3.88 Impact Factor
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- "Janthinobacterium spp. are Gramnegative , motile bacteria that have been isolated from a wide range of habitats including the Antarctic (Mojib et al., 2011), as symbionts of insects (Zhang et al., 2011a) and amphibians (Brucker et al., 2008; Harris et al., 2009), and from plants and the environment (Kang et al., 2007; Kim et al., 2012). Janthinocin A, B and C were isolated in 1990 from a water isolate of Janthinobacterium lividum (O'Sullivan et al., 1990). "
ABSTRACT: The current crop of antibiotics in clinical use are either natural products or their derivatives. However, the rise of a multitude of different antibiotic resistant human pathogens has meant that new antibiotics are urgently needed. Unfortunately, the search for new antibiotics from traditional bacterial sources often results in a high rediscovery rate of known compounds and a low chance of identifying truly novel chemical entities. To overcome this, previously unexplored (or under investigated) bacterial sources are being tapped for their potential to produce novel compounds with new activities. Here, we review a number of antibiotic compounds identified from bacteria of the genera Burkholderia, Clostridium, Lysobacter, Pantoea and Xenorhabdus and describe the potential of organisms and their associated metabolites in future drug discovery efforts.International journal of medical microbiology: IJMM 09/2013; 304(1). DOI:10.1016/j.ijmm.2013.08.011 · 3.42 Impact Factor
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ABSTRACT: Psychrophiles thriving permanently at near-zero temperatures synthesize cold-active enzymes to sustain their cell cycle. Genome sequences, proteomic, and transcriptomic studies suggest various adaptive features to maintain adequate translation and proper protein folding under cold conditions. Most psychrophilic enzymes optimize a high activity at low temperature at the expense of substrate affinity, therefore reducing the free energy barrier of the transition state. Furthermore, a weak temperature dependence of activity ensures moderate reduction of the catalytic activity in the cold. In these naturally evolved enzymes, the optimization to low temperature activity is reached via destabilization of the structures bearing the active site or by destabilization of the whole molecule. This involves a reduction in the number and strength of all types of weak interactions or the disappearance of stability factors, resulting in improved dynamics of active site residues in the cold. These enzymes are already used in many biotechnological applications requiring high activity at mild temperatures or fast heat-inactivation rate. Several open questions in the field are also highlighted.01/2013; 2013(2):512840. DOI:10.1155/2013/512840